Capillary electrophoresis

Agarose Gel Electrophoresis Since the discovery of the four classes of macromolecules (proteins, lipids, carbohydrates, and nucleic acids), researchers and biochemists have fine-tuned methods that reveal structural information about each of them. Different structural properties determine different functions and chemical properties, and scientists have developed intricate techniques to observe, quantify, and isolate molecules based on their aforementioned properties. Agarose gel electrophoresis,…

performing a DNA extraction and purifying your template. The purification will be done in a QIAquick column and it will remove dNTPs and primers from your PCR reactions that created your amplicon. Determine the quality of your DNA by running a gel- electrophoresis and a Spectrophotometry of your sample. The thermos cycler begins the reaction by heating the two DNA strands to 90 Celsius in order to separate them. Then the primer anneals to the sequence at a lower temperature of 54 Celsius. Then…

breakthroughs in the field of Molecular biology (1). The first efficient method to sequencing DNA was found by F.Sanger in 1977 (2). But the huge desire to sequencing genomes required faster methods and many improvements were made. Fluorescence, capillary electrophoresis and microarrays led to a new way for sequencing genes : the Next Generation Sequencing (NGS). How do Sanger technique and NGS work ? What are their applications? Sanger sequencing The Sanger technique is based on the…

Gel electrophoresis is a method used for separation and analysis of molecules such as DNA, RNA, and proteins, based on their sizes and polarity. DNA (deoxyribonucleic acid) is a molecule that carries most of our genetic information, and possesses a negative charge. During gel electrophoresis, DNA fragments can migrate through the gel also known as agarose when placed in a powerful electrical field. The rate at which the DNA fragments will move through the gel depends on their relative size.…

The Specimen Reception – where I spent most of my day-to-day work – is part of the Lab-Med group that is situated in the Central Pathology lab, and is made up of the biochemistry laboratory, the haematology laboratory – which includes cryobiology and coagulation, the microbiology lab, the histopathology lab – which includes cytopathology, the immunology lab, cancer molecular diagnostics, and transfusion medicine, as well as the Irish mycobacterium reference lab, and the national MRSA reference…

can be also be detected in surface water, waste water treatment plants2 and in banknotes3. Different methods have been reported for cocaine detection; enzyme immunoassay3, 4, 5, 6 , gas chromatography (GC) and liquid chromatography (LC), capillary electrophoresis (CE), immunoassay, thermal desorption tandem mass spectrometry (TD-MS2) and ion-mobility spectrometry (IMS)3.…

Preliminary qualitative chemical tests show the presence of alkaloids, carbohydrates, saponins, steroids, terpenoids, phenolics, coumarins and fixed oil, etc.18 Desorption electrospray ionization was employed for fast and direct ambient detection of the anti-tumor drug, camptothecin, and its derivative, 9-methoxycamptothecin in Nothapodytes nimmoniana J. Graham. Different parts of the plant such as leaves, stems and bark were examined. The…

Chromatography is a method of separation in which the components of a substance are separated and distributed between two phases, which are the mobile phases and stationary phase (1). There are multiple types of chromatograph, which includes paper chromatography, column chromatograph, retention chromatography, gas chromatography, liquid chromatography etc. Each technique has its own strengths and weaknesses, but all are useful in getting separations of different components (8). Since many…

of cerebral hypoxia from poor tissue perfusion.” Poor perfusion of oxygen in body systems due to decreased levels lead to further symptoms which include increased heart murmurs, heart rate, low blood pressure, decreased pulse pressure, delayed capillary refill, jaundice, ulcers, Chronic Kidney Disease, and dark yellow or amber colored urine. (Ignatavicius & Workman, 2013). Many of these later symptoms occur simultaneously in the body systems and are more common during acute pain episodes.…

Other methods, such as laser desorption, anionic dyeing, and capillary electrophoresis are used in forensics, however for this experiment paper chromatography worked well because of its simplicity, low cost, and ability to be adjusted. Throughout this lab, the group will test various solutions to conduct paper chromatography…