Capillary electrophoresis

and varying types of supporting media can also be used, both of these are demonstrated throughout the practical sessions. The first practical used the combination of a vertical gel and polyacrylamide media, this is known as polyacrylamide gel electrophoresis (PAGE) and has a key role in protein analysis. Both D1S80 and D3S1358 have been shown in numerous papers, with the papers by Limborska et al (2011) and Momhinweg et al (1998) showing particular correlation to the first experiment…

be analyzed from a variety of human samples including blood, semen, saliva, urine, hair, buccal (cheek cells), tissues, or bones. The polymerase chain reaction (PCR) is used to amplify the genomic DNA from a sample and electrophoresis is used to arrange the segment.…

To examine the DNA, a gel electrophoresis test needs to be run. The first step of gel electrophoresis is to set the gel, which is made of agarose. Agarose is used to separate DNA molecules, and acrylamide is used to separate proteins. The gel starts off as a liquid, which is poured into a molding tray and after it sets, it will turn into a solid. Next .all of the DNA needs to be collected and copied with a Polymerase Chain Reaction, PCR, which is vital to the success of the gel process because…

In this experiment Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) was performed under reducing conditions to estimate the molecular weight of proteins within a sample. The protein sample was from the previous using anionic exchange chromatography to partially purify N-acetyl--D-hexosaminidase, Lab 3. The molecular weight was determined through the interpolation of a standard curve of proteins with a known molecular weight. The molecular weight of band 1 was determined to…

The technique used in this experiment was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small…

Bio Lab- Denaturing Proteins Period B- Ms. Dann October 8, 2014 Nick D’Ambrosio, Matt Hermann, Carly Pruitt Title: Electrophoresis and Protein Weight Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel. Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to…

Bovine serum albumin (BSA) is usually used for generation of nanoparticles (NPs) in drug delivery systems. This paper presents a study on the simple coacervation method for fabrication of BSA nanoparticles.An artificial neural network (ANN) model was developed to predict the diameter of nanoparticles based on experimental data. Effect of pH, temperature, initial BSA and cross linker concentration, molar ratio of BSA to ethanol, agitation rate and rate of ethanol addition were investigated on the…

RESULTS Lipid Extraction The prediction made was that adipose tissue most likely had the highest lipid content and skeletal muscle had the lowest lipid content when comparing liver, muscle, and adipose tissue. The percent lipid was found to be 30% in liver tissue, 70% in muscle tissue, and 90% in adipose tissue in the samples collected. Protein Extraction and Quantification The absorbance vs concentration curve prepared from the Bradford Protein Assay standards was found to have a linear…

the DNA in its membrane. Later some Elution Buffer was used to separate the DNA from the membrane of the spin column. Day two consisted of making the plasmid DNA resistant to Kanamycin and Ampicillin. Day three we made 0.8% agarose gel and ran electrophoresis to be able to see the weight of the DNA. Day four we genetically transferred the ligated plasmid DNA to E.coli and plated the cell mixture. Day five we looked at our results and went over them. All of this works together for bacterial…

Article Comparisons to Southern (1975) Article One The article “Detection of specific sequences among DNA fragments separated by gel electrophoresis” (1975) by E.M. Southern focuses on the way that the fragments of DNA can be transferred from agarose based gels to cellulose nitrate filters. Then fragments themselves are hybridized to active RNA. E.M. Southern’s main influences for the investigation were the studies of Smith and Wilcox (1970), and Kelly and Smith (1970), which showed that the…