Capillary electrophoresis

Agarose gel electrophoresis of DNA is used to seperate mixtures of protein and nucleic acid fragments. Molecules tend to differ in size, charge, and mobility. The objective of this procedure is to predict the distance of DNA migrating towards the positive electrode in the electrophoresis chamber. Many scientists use agarose gel electrophoresis to examine the DNA of criminal suspects. The first step in this procedure is to prepare a buffer containing 1% of the liquid agarose gel. Once the gel…

vaccines, such as the influenza or polio vaccines are examined, purified and improved, gel electrophoresis. Due to confidential reason, the exact process of vaccines analysis are unknown, however it is suspected that the steps are similar to DNA analysis by electrophoresis and medical reports have claimed that electrophoresis presents an effective method of vaccine analysis (ehow). Through gel electrophoresis, scientists are able to identify the types of bacteria that are antibiotic resistant…

reason could be that the cell did not get enough heat shock, or not enough pre-incubation time with CaCl2, which means the bacterial cell wall was not permeable enough for the DNA to come inside and interact with the bacterial DNA. The DNA gel electrophoresis showed that there were no fragments…

In this experiment, a SDS-PAGE gel was used to analyze the protein samples from the MBP-AP and WT-AP experiments. The samples are then referenced to the ladder to determine the molecular weight of the MBP-AP and WT-AP proteins. Then the UV absorbance of the two proteins from 240 nm to 340 nm is determined using a nanovolume cuvette. The absorbance at 280 nm was then used in conjunction with data from previous experiments to determine the concentration of the MBP-AP and WT-AP protein samples.…

PCR amplification of CO1 mitochondrial gene. DNA samples were assessed for presence and quality using agarose gel electrophoresis. Species identification was performed by importing finalized CO1 sequences into two DNA databases to ensure accurate identification. Phylogenic analysis was then conducted to determine relationships between identified species. Electrophoresis Electrophoresis of DNA run in 2% agarose gel showed successful extraction of DNA in samples 1-8, 12 and 14-16. Samples were of…

Gel electrophoresis is used to separate DNA, RNA, and protein molecules by using an electric field. Protein gel electrophoresis is similar to agarose gel electrophoresis, but runs protein bands instead. The technique of protein gel electrophoresis uses cross-linked polymers of acrylamide (which is a neurotoxin and requires careful handling) or polyacrylamide to separate proteins and small nucleic acids. This technique takes longer than the agarose gels used to separate larger nucleic acids. Also…

the transformation of the ade2 gene to the kanamycin resistant gene (ade2::kan2R) cause the cell to become red and grow on mediums containing G418 or kanamycin resistant mediums was observed to have occurred. PCR and Gel Electrophoresis: Figure 1 is the product of gel electrophoresis containing the wild type and transformed PCR products, either being or not being cut by HindIII. From the left (reader’s left) of the gel to the right the lanes are; 1.Ladder, 2. transformed PCR product, 3.…

cell lysis buffer (). Next, the proteins from the healthy volunteers and the sick volunteers will be stained with CyDye Cy3 and Cy5, respectively, and combined into one solution. The resulting solution will then be subjected to 2-dimensional gel electrophoresis. This will be done by transferring the mixed protein solution to isoelectric tube gel (pH 3-10) and subjecting it to a constant electric current (145mA). After the gel has been allowed to run for about an hour, the isoelectric tube will…

Denaturing Protein Gels Kaytlin Goodwin Cedarville University Introduction Electrophoresis experiments are conducted on proteins for the purpose of separating proteins based on their characteristics. The characteristics that can be examined during the experiment are dependent on the preparation of both the proteins and the gel medium through which they will run. Native gel electrophoresis experiments highlight and examine multiple protein characteristics, such as size, charge, and…

Sweta Soni 2/22/15 Biochem Lab Experiment 5 Introduction The purpose of Experiment 5-1 is to separate a mixture of three molecules—blue dextran, cytochrome c, and potassium chromate—through the use of gel-filtration chromatography. The volume at which each molecule elutes will be recorded in order to determine the accuracy of separation. The purpose of Experiment 5-2 is to purify the E. coli enzyme, β-Galactosidase through the use of affinity chromatography on…