Calorie restriction

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    Introduction: In this lab experimentation, the group conducted Deoxyribonucleic acid isolation and restriction analysis on a plasmid from Escherichia coli cells. Plasmids are small circular DNA that are in the bacterium cells. Escherichia coli is a gram- negative bacterium that is known for variable reaction to antibiotics, and can be genetically manipulated. The gram- negative bacterium, Escherichia coli can be genetically manipulated by extracting a certain plasmid that allows it to resist…

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    contain the desired sequence, restriction enzymes are used. Restriction enzymes can recognize a specific sequence of nucleotide and hydrolyze the bond of the DNA backbone. Hence, the fragments from the same DNA source will also be the same if cleave by the same restriction enzymes. Restriction enzymes will recognize specific palindrome sequence and cleave there. The number of fragments and sizes of the fragments obtained by cleaving the dsDNA are dependent on the restriction enzyme sites and the…

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    coli (E. coli) by isolating and purifying a plasmid from E. coli, determining its orientation with respect to the plasmid backbone by restriction mapping, and transforming it into an alternate E. coli strain. Methods: Each individual received either E. coli A or B strain of pKN800 plasmid DNA. The plasmid was purified and isolated, then cut with PstI restriction enzyme to create a sample of PstI-cut and uncut pKN800 plasmid DNA and to distinguish orientation A from B. These samples were loaded…

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    Preparing Eppendorf Tubes

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    Restriction Mapping of Plasmid DNA Preparing 5 Eppendorf Tubes All tubes contain 1 μL of the plasmid DNA, and 2 μL of 10X buffer #2. Tube 1 is the control so there is no enzyme added only 17 μL DI water. For tube 2 the DNA is mixed with 0.5 μL of BamHI and 16.5 μL of DI water, to see total number of base pairs from the linear DNA. In tube 3 DNA is digested with 2 restriction enzymes, which can give the relative distance between the two enzymes. To be more specific, 0.5 μL of Bam HI and EcoRI…

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    Restriction Enzymes

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    1. Where do restriction enzymes come from and what is their original purpose? Restriction enzymes come from bacteria. Bacteria used restriction enzymes to cut up DNA into fragments to fight off viruses. 2. a. Why do you get different fragment sizes if you cut the DNA from two different species with the same restriction enzyme? Each species has a different arrangement of bases, and the restriction enzyme only cuts after a specific order of bases. Due to that being the case, the specific…

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    Due to limited time constraints and having lab only once a week this lab was broken up into five periods, the last one specifically to go over the results. Day one consisted of extraction of plasmid DNA, which we used Resuspension Buffer to break up our pellet after removing the supernatant (liquid) after centrifuging our overnight culture. Then Lysis Buffer was used to break down the membrane of the cells so that we could then add Neutralization Buffer to bring our sample from basic to neutral…

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    Alibrio Ficheri Lab Report

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    A. fischeriThe overall purpose of this study was to create a genomic library of Aliivibrio fischeri (A. fischeri) thus aiding in creating a restriction map of the lux operon. It also employs typical molecular techniques important for biologists to understand. In this portion of the lab, the chromosomal DNA (chDNA) will be isolated. Its purity will be measured using spectrophotometric analysis. Lastly, the DNA will be digested and verified via gel electrophoresis. A. fischeri is a gram…

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    Biotechnology plays a vital role in today’s society. According to Webster, biotechnology is the manipulation of living organisms or their components to produce useful, usually commercial products. Biotechnologies can vary in applications and complexity, but they all have the ability to change our society and help us prepare for and meet some of the world’s most pressing challenges today. In the history of uses of biotechnology, it has been known to help provide a more reliable food source,…

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    really important in DNA fingerprinting. However, there could be one in sixty-four billion chance that two unrelated entities share the same genetic code. DNA fingerprinting is consists of many common procedures: RFLP (Restriction Fragment Length Polymorphism) utilizes special restriction enzymes to cut portions of the extracted DNA and focuses on repeating sequences of the DNA bases that differs from each individual. Electrophoresis separates the DNA and measures the portions by length.…

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    Vaccine Analysis Case

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    Discussion When comparing the banding patterns of the crime scene to those of the suspects, the resulting gel indicates that Suspect 2 was at the scene of the crime. Although enzyme 1 produced identical DNA fragments across the gel, enzyme 2 did not. This is evident in lane D and possibly indicates that this enzyme was unable to bind to recognition sites similar to the crime scene DNA in well B. Thus, it produced a DNA fragment smaller in size that travelled further. Since the DNA evidence in…

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