Calibration

Introduction According to the Food and Drug Administration’s (FDA) final regulation (21 CFR Part 120) that requires a processor of juice to evaluate its operations using Hazard Analysis Critical Control Point (HACCP) principles. Under HACCP principle 7, it is required to establish procedures for verifying the HACCP system is working as intended. This extension bulletin gives guidelines to establish and manage an effective verification program. Outline 1. Importance of verification.…

Introduction: Within this task we were required to determine the ecological state of the Reddam House wetland, which I presume is at a good standard. I decided to test the ecological status of the wetland by gathering samples from the wetland and testing it for various aspects in the water and the plants that are found within the area. Method: • Collected water in a sterilized bottle at Reddam House wetland as well as observed surrounding fauna and flora. • Took the water to Rand Water to have…

During this experiment that was conducted in partners, the concentration of RNase H that was purified in the previous experiment #6 was determined and also the concentration of an unknown given solution using their absorbance values obtained from the spectroscopy apparatus. These concentrations were calculated using the equation of the line produce with the values of the concentration (x-axis) and corresponding absorbance (y-axis) of five protein bovine gamma globulin standard solutions. In…

of Euclid’s Deep Survey is 1. to investigate galaxy formation with very high statistical confidence. 2. to obtain spectroscopic redshifts required to calibrate the photometric redshifts needed for the weak lensing experiment. 3. to support the calibration of the visible PSF (point spread function, i.e. the size of a point source) by repeatedly visiting the same fields on the sky. A small PSF is vital for the weak lensing This survey will be 2 magnitudes deeper than the wide survey, by…

While it may take time to prepare the standard solutions of known concentration to develop the calibration curve for the spectrophotometer before testing a filtrate, the equations involved in determining the amount of a compound necessary for preparing solutions of specific volume and concentration are simple and do not take a large amount of time to…

To begin the experiment, the Spec 20 was turned on to warm up for 15 minutes and set to maximum absorbance wavelength. Ten test tubes were then prepared. The Spec 20 was set to 0% and a test tube of blank solution (water) was wiped off and placed into the Spec 20. Transmittance was then set to 100% to calibrate the machine. Next, five test tubes were prepared and labeled with one of the standard solutions of 0.05 M, 0.1 M, 0.2 M, and 0.5 M CuSO4 in them. The Spec 20 was set to 600 nm and %…

Introduction Equilibrium is the concept that in a chemical reaction where both the forward and reverse reactions occur, equilibrium is reached when both reactions are occurring at the same rate, thus once a reaction is at equilibria both the forward and reverse reactions are occurring at the same rate. For a given Chemical Equilibrium at a given temperature, keq can be calculated that will be constant no matter the initial values of the reactants and products. For a reaction: the equilibrium…

The pulverized sample (1.0 g, 60 mesh) was extracted with 20 mL of the 80% methanol for 24 h in a shaking incubator at 25 °C. The crude extract was filtered through a PTEE syringe filter before UPLC analysis. for the calibration curve, the peak area of the isolated phenolic compounds was integrated with the UPLC chromatogram at 335 nm and plotted against the concentration to create a linear curve. The stock solution was prepared in MeOH to obtain a 1 mg/mL concentration…

Through this experiment we measured how fast a chemical reaction occurs, by changing the degradation rate in Albumin when added to different enzymes. Albumin is a protein found in egg white, which is considered to have important storage and nutritional functions. Albumin has also been used in medicine to treat heavy metal intoxication. We ran Albumin through four different conditions to see which would make the Albumin degrade faster. We predicted that the Albumin would degrade with pepsin the…

Introduction and Social Relevance 2015 was a tragic year for South Australia. 102 people died on the roads and over 750 serious injuries occurred in South Australia alone. 44 of the fatalities were young men and women who died due to driving over the allowed alcohol consumption limit of 0.05 (Responsibility, 2016). This did not however need to be the case, these fatalities would not have occurred if the drivers were tested and stopped beforehand. Over 600 000 drink driving tests occurred last…