Calibration curve

mL acetate buffer (30 mM; pH 3.6), 2.5 mL TPTZ solution (10 mM) and 2.5 mL ferric chloride solution (20 mM). The mixture was incubated for 15 min at 37 °C before use. Ascorbic acid (vitamin C) was employed as a standard in this assay, and its calibration curve was obtained by using its concentrations ranging from 50 mg/L to 500 mg/L in water. To 2.85 mL FRAP reagent in a test tube, 150 μL plant sample (0.1 mg/mL, in methanol) or standard was added. The mixture was incubated for 30 min in the…

recorded by placing each sample into the calibrated Spec-20 spectrophotometer. Transmittance was found by dividing percent transmittance by one hundred. The absorbance value was calculated using the equation A=-log(T) and was graphed to create a calibration…

Let’s start the introduction by talking about our main target, Escherichia coli or how it is mostly unknown, E.coli, are a diverse and large group of bacteria found food, the environment, and in the intestinal tracts of humans and animals were it normally inhabits and it can also cause infection in other parts of the body, especially the urinary tract. Its name was given by a German physician called Theodor Escherich and it is the most common member of the genus Escherichia. E.coli is “a…

wavelength. The standard solution of Decitabine was prepared and was injected by changing anwavelength along with the optimized method. LOD and LOQ[17] Limit of detection (LOD) and limit of quantification (LOQ) of Decitabine was calculated from the calibration curve method. The linearity solutions of Decitabine were prepared and injected. LOD and LOQ were calculated by using following…

In this investigation, the mass (g) of a beaker and a graduated cylinder were calculated and recorded into a lab notebook. The density of distilled water was calculated without any NaCl by measuring the mass of a sample of water in a beaker, and then subtracting the mass of the beaker to leave the mass of water. This measurement was recorded into a lab notebook in Table 1 under “Total Mass of Solution” and “Mass of solution”. The contents of the beaker are then transferred to a graduated…

was the salivary amylase concentration. The salivary amylase concentration was obtained through various procedures including, PCR reactions to amplify the DNA, measuring wave frequency of a DNA sample using a spectrophotometer, and calculated calibration curves. There were also controlled variables, including the amount of substances we would mix together, or the amount of PCR cycles the DNA samples underwent to amplify the DNA. Having controlled variables helps ensure there is less variability…

Introduction: The goal of this lab was for students to learn how to use a spectrophotometer to find the absorbance of four known copper (II) sulfate solutions, four copper (II) sulfate solutions that were created by dilution, and one unknown copper (II) sulfate solution. The purpose of this experiment was to help students learn how to use the Spec-20, which is a machine that measures the transmittance and absorbance of a solution, learn about the linear relationship between absorbance and…

4.0 Results and Discussion Before the strontium quantification of the solutions resulted from the ion exchange experiments, it was necessary to investigate what is the range where strontium absorbs radiation linearly and what is the detection limit. The graphs below show the absorbance (atomic absorption) in function of the strontium concentration and it can clearly be seen in figure 2 that at concentrations above 10 ppm the samples start to present non-linear absorption. Therefore, all the…

low stiffness material but in case of low speed we should always choose high elastic moduli probe. Adhesion force measurement: Adhesion force measurement can be done during recording of the force vs displacement curve. This measurement has to be done in force calibration way. In the below plot point A indicates that the distance between the tip and the sample is too high, hence there is no interaction between sample and probe. After that, probe approaching the sample surface and…

an understanding of the relationship between chemistry and food products while developing and enhancing good lab techniques. We applied dilution concepts to carry out the experiment. By using these concepts and techniques, we created a dilution curve to display and understand our data. Procedure1 Materials The materials used in this experiment are whole grain cereal, Sodium Acetate, water, hydrochloric acid, 2-2 Dipyridyl solution, Hydroxylamine-HCl, and D.I. water. The glassware and…