Calibration curve

As indicated in Table 1, the maximum wavelength was at 625 nm at 1.063 absorbance. The absorbance values from cuvettes #1 through #7 were recorded at 625 nm to create the concentration curve Figure 2. The concentration curve is conducted at the maximum wavelength to produce the optimal results for the diluted concentrations of Fast Green.The purpose of the graph is to locate the unknown; Beer-Lamberts law, the concentration is proportional to the absorbance…

To begin the experiment, the Spec 20 was turned on to warm up for 15 minutes and set to maximum absorbance wavelength. Ten test tubes were then prepared. The Spec 20 was set to 0% and a test tube of blank solution (water) was wiped off and placed into the Spec 20. Transmittance was then set to 100% to calibrate the machine. Next, five test tubes were prepared and labeled with one of the standard solutions of 0.05 M, 0.1 M, 0.2 M, and 0.5 M CuSO4 in them. The Spec 20 was set to 600 nm and %…

Chemicals: Folin-Ciocalteu reagent, sodium carbonate, gallic acid, trifluoroacetic and sulphuric acids, Trolox (6-hydroxy -2, 5, 7, 8- tetramethyl chromane-2-carboxylic acid), ABTS (2, 2'-azinobis (2-methylpropionamidine) dihydrochloride), 2′, 7′-Dichlorodihydrofluorescein diacetate (H2DCFDA), dexamethasone (DEX), and lipopolysaccharide (LPS) were purchased from Sigma Inc. (St. Louis, MO). Catechin, epicatechin, procyanidin B1 and B2 were purchased from Chromadex (Irvine, CA). Dulbecco’s…

showed that it is a cysteine protease. Preliminary investigations such as effect of pH and temperature on the caseinolytic activity of crude protease were done. Stability towards temperature and pH were also evaluated. The pH and temperature activity curves and other stability studies suggested the possible presence of one proteolytic enzyme in the latex of Vallaris solanacea. In the present study, separation and purification of the cysteine protease…

Introduction Equilibrium is the concept that in a chemical reaction where both the forward and reverse reactions occur, equilibrium is reached when both reactions are occurring at the same rate, thus once a reaction is at equilibria both the forward and reverse reactions are occurring at the same rate. For a given Chemical Equilibrium at a given temperature, keq can be calculated that will be constant no matter the initial values of the reactants and products. For a reaction: the equilibrium…

51 USP Tailing 1.38 1.32 1.18 4.3.1.2 Linearity A linear correlation coefficient factor was obtained between the concentrations of Lamivudine, Zidovudine and Nevirapine versus the peak area. The calibration curves were linear for concentrations between 15-150 µg/mL. The linearity of the calibration curves was evaluated by the values of the correlation coefficients (r2). The correlation coefficients were 0.999 for Lamivudine, 0.999 for Zidovudine and 0.999 for Nevirapine. The results of the…

Abstract For this experiment the amount of zinc in vitamin water had to be determined. To do this, three methods were applied. The first was by a calibration plot method, where zinc standards were prepared and from the absorbance of those standards a calibration curve was plotted, which in turn was used to determine the amount of zinc in vitamin water. The second method was standard addition. For this method, an un-spiked and spiked solution were prepared. The un-spiked solution only contained…

Graph 4 demonstrates the correlation between the transmittance and concentration levels of KMnO4, in how as the levels of concentration increase, the transmittance decreases. The experiment during week 2 was very similar to week 1 except we used potassium phosphate instead of potassium permanganate. Table 3 demonstrates the effect of wavelength on T% and absorbance. The same equation used for Table 2 was used in Table 3 in order, to calculate the absorbance. The highest absorbance level was…

acids with a strong base are studied by observing how the pH of the solution changes as the titrant is added. A plot of pH versus amount of titrant added will be constructed. Such a curve is called a pH titration curve. The equivalence point of the reaction will be determined from an analysis of the pH titration curve, and by the use of acid-base indicators. The pH measures the solution whether it is acidic and basic. The pH of the solution could be determined by using the following equation,…

Efaverinz+Polysorbate-80 IR spectra of Efaverinz+PEG-400 IR spectra of Efaverinz+MCC+AEROSIL Fig no: 10 IR spectra of the Optimized Formulation Table 10: STANDARD CALIBRATION CURVE FOR EFAVERINZ IN 6.8 PHOSPHATE BUFFER of 2% SLS: CONCENTRATION (µg/ml) ABSORBANCE (nm) 0 0 2 0.077 4 0.138 6 0.211 8 0.281 10 0.352 Figure 11:Standard calibration curve for…