Calibration curve

Introduction: Resistance to antibiotics is an ongoing concern of the pharmaceutical industry. The limited number of antibiotics in the market to treat Gram negative bacteria made their resistance an issue that is being addressed worldwide. The isolation of colistin from Bacillus polymyxa var. colistinus in Japan in 1949 and the introduction of colistin into the market in 1959 as its inactive prodrug colistin methanesulfonate (CMS) were significant milestones in the treatment of this type of…

analyzed by Shimadzu FTIR spectrometer (from400 to 4000 cm-1) at room temperature. pH values measured by pH meter (HANNA). Perkin Elmer Lambda 35,UV–vis spectrophotometer was used for determining [MB] and [CR] samples employing the standard calibration curve of MB and CR at the maximum wavelengths 665 nm and 495nm,…

The chromatography was performed on an ACQUITY TM UPLC system (Waters Corp., Milford, MA, USA). The UPLC system included quaternary solvent manager, a binary pump, degasser, autosampler with an injection loop of 10 μL and a column heater-cooler. The separation was performed on Acquity UPLC BEH TM C18 column (50 × 2.1 mm, i.d., 1.7 μm, Waters, USA) maintained at 40 °C. a mobile phase of acetonitrile containing 0.1% formic acid and aqueous 5 mM ammonium acetate buffer containing 0.1% formic acid…

Figure 1 shows the relationship between concentration and absorbance known as the Beer-Lambert law or calibration curve. The data from the made solutions in table 2 was used to create the graph. The graph has a linear trend line that predicts where a point would fall on the graph. The equation of the trend line is equation 7: y=0.9508x-0.0055 with a R2 value of 0.99943. This equation was used to find the true concentration of the made solutions as well as the unknown solution. The R2 value…

Introduction Chromatographic process is a separation technique which has to do with the mass transfer of test sample between a stationary and mobile phase. HPLC (High Performace Liqud Chromatography) is a vast system which involves the use of a mobile and stationary phase to separate materials. The stationary phase is usually a column packed with solids (usually silica gel) while the mobile phase is usually a solvent or a mixture of various solvents. The mobile phase usually, is the carrier of…

moldm-3, 0.030 moldm-3, 0.035 moldm-3, 0.040 moldm-3, 0.045 moldm-3, 0.050 moldm-3, 0.055 moldm-3) and a colorimeter, find the absorbance for each of the concentrations. This can then be used to to plot a calibration curve (concentration…

UPLC analysis. for the calibration curve, the peak area of the isolated phenolic compounds was integrated with the UPLC chromatogram at 335 nm and plotted against the concentration to create a linear curve. The stock solution was prepared in MeOH to obtain a 1 mg/mL concentration. Ten curves were obtained by diluting the individual stock solutions in MeOH to six different concentrations including 0.5, 1, 2.5, 5, 50, and 100 μg/mL. The correlation coefficients (r2) of each curve were observed…

55 1651.67 C=O Stretching 2. 1416.96 1416.98 C=C Stretching 3. 1072.39 1072.37 C-O Stretching Figure 7.4: FTIR spectra of Prazosin + HPMC 7.2.2. Preparation of Calibration curve of prazosin hydrochloride Determination of λ max of prazosin hydrochloride Exhibited peak absorbance at 330nm (λ max) in methanol. Standard solution 50mg of standard drug was accurately weighed and placed in 250ml of volumetric flask and…

merits a fairly successful model. We had to deal with one important constraint: we are working with data specific to a first-order system, and must use equations that appropriately match. Our algorithm inputs data representing a cooling or heating curve and outputs four parameters: yL, yH, ts, and � (calculated by the prior 3 parameters). We compare � of each FOS system to find the most effective system. The first design choice we made was how we determined ts. ts is best represented when there…

Introduction: The roles of ATP and creatine in muscle contraction The store of ATP in muscle tissue is limited even though it is the immediate source of energy for everything we do including muscle contraction. The cellular ATP needs to be regenerated from ADP quickly in order to keep the contraction going otherwise the vertebrate muscle contraction will use up the present ATP in 1 second. Fortunately, most cell has a high rate of ATP “turnover” which means ATP can be swiftly synthesised from…