Temporal analysis of gene expression in the embryonic murine Schwann cell lineage 1Anjali Balakrishnan, 2Morgan G. Stykel, 1,2Yacine Touahri, 2*Jeff Biernaskie, 1*Carol Schuurmans Institutional Affiliations: 1Department of Biochemistry and Molecular Biology in the Cumming School of Medicine, 2Department of Comparative Biology and Experimental Medicine in the Faculty of Veterinary Medicine, both in the Alberta Children’s Hospital Research Institute and Hotchkiss Brain Institute, University of…
Transcription is the process in which DNA is converted to RNA. Ribonucleic Acid (RNA) is a polymeric molecule. RNA is involved in protein synthesis, and it is sometimes even in the transmission of genetic information. The main job of RNA is to transfer genetic information that is needed for the creation of proteins from the nucleus to the ribosome. Without RNA proteins could never be made (News-Medical). Transcription is the process of making an RNA copy of gene sequences. This particular copy…
It is expected that if the concentration of sucrose increases from 0.0 M, 0.2 M, 0.4 M, 0.6 M and 0.8 M (±0.01 g) in a 40 (±0.5) mL sucrose solution, then the average percent change in mass in Solanum tuberosum core will decrease. This is because if the Solanum tuberosum cores are placed in a hypotonic sucrose solution, which is described as having lower osmolarity, than the percent change in mass of the Solanum tuberosum core will increase due to the fact that water molecules will be moving…
is a part of a series of connected 'hallways' that carry materials to various parts of the cytoplasm. The RER has many ribosomes attached to it and it is tasked with creating proteins. The Smooth Endoplasmic Reticulum(SER) is also a part of connected 'hallways that transport materials. However, instead of creating proteins, it has no ribosomes and it instead is the place where lipids…
Genomic engineering is the process of modifying specific sites in DNA sequences of cells or organisms. The ability to achieve effective and precise editing of the genome has been achieved and advanced through the development of sequence-specific nucleases1. Zinc-finger nucleases and transcription activator-like effector nucleases (TALENs) are programmable DNA binding nucleases previously used to achieve this targeted editing of DNA. However these tools have been found to be both time consuming…
5. Normal muscle contraction occurs in a series of steps, and they are as follows: First, a motor neuron must have acetylcholine released in the synaptic cleft. The acetylcholine is then able to bind with receptors found in the cell membrane. This binding causes the Ca+/Na+ channels to open. The terminal cisternae then releases calcium into the muscle fiber. Calcium will begin to bind to troponin. The troponin moves tropomyosin out of the way since it blocks the actin’s active site. Next, actin…
that affects genetically predisposed individuals across the world. The genetic predisposition occurs on HLA haplotypes DQ2 and DQ8, and is present on autosomal chromosome 6. Celiac disease is induced by the consumption of foods that contain the protein gluten, which affects the auto-antigen tissue transglutaminase. Gluten, a 33 amino acid peptide high…
Agarose gel electrophoresis is a lab technique that is used to separate and identify DNA and RNA according to the size of the individual molecule fragments. It has many common uses in modern molecular biology such as separation of restriction enzymes, analysis of PCR results, the number of DNA in a sample, DNA sequencing, DNA finger printing, and forensic science. The basis behind agarose gel electrophoresis is manipulating the highly negative charge held by DNA. When placed in an electric…
wellspring of protein, sugars, and fiber. Lentils are thought to be a boring protein. Part green peas can be additionally included into an indistinguishable class from lentils. Sustenance: 1 container cooked lentils = 18g protein, 1 measure of green peas = 8g protein *Quinoa is a without gluten grain (in fact a seed) that is utilized as a starch. It's viewed as a starchy protein since it contains starches and in addition protein and fiber. Nourishment: 1/2 container cooked quinoa = 7-9g protein…
of these elements is dependent on brain region, subcellular location, age, species, genetic and environmental factors (Prohaska, 1987). These elements are considered as cofactors, catalysts and structural components for many cellular enzymes and proteins in the CNS. They also play roles in the homeostatic, regulatory mechanisms maintaining their concentrations in the CNS (Richardson and Ponka, 1997). They take part in neuronal activities such as neurotransmission, synaptogenesis, neurogenesis,…