C-reactive protein

This means finding proteins that express affinity and specificity for the respective ligand. According to Ewald (2011) there are three good options, CitX (periplasmic binding protein from Salmonella typhimurium) and the binding domains of DpiB (sensor histidine kinase from E. coli) and CitA (sensor histidine kinase from Klebsiella pneumoniae). We would then purify all three proteins and record fluorescence energy values when bound to citrate for future…

The purpose of this experiment is to examine the extraction of the DNA material that was extracted from the Chromosomal DNA from E coli in laboratory 9. DNase is used to help extract the Sugar phosphate backbone of the DNA. DNase should only cleave DNA not RNA but DNase can classify contaminated RNA from chromosomal DNA. Methods A. preparation :E. coli was used to extract the DNA as instructed in laboratory 9 to use in the experiment on analyzing in this experiment. There was 500 μL of the…

The simple definition of Eukaryotes is that they are any organism whose cells contain a nucleus and other membrane enclosed organelles. Taxonomically, Eukaryotes belong to the taxon Eukarya. The three types of eukaryotic microbes are fungi, protozoa, and algae. Because the three types of eukaryotic microbes are all composed of eukaryotic cells, they have basic similarities in cellular structure, including the presence of a nucleus. However, these types of microbes do differ in many significant…

gain in function by the protein. When this occurs, the TDP-43 protein has usually shifted its location from the nucleus to throughout the cytoplasm of the cell. This abnormal accumulation of TDP-43 in the cytoplasm is not only found in neurons and glial cells of the primary motor cortex but as well as in brainstem motor nuclei, the spinal cord, and in certain associated white matter tracts (Mackenzie et al 2010). It is at this point that TDP-43 becomes phosphorylated and the C-terminus cleaved…

α-Lactalbumin, a multifunction protein in milk, forms an enzyme complex with galactosyltransferase that catalyzes the last reaction in lactose synthesis.1 α-Lactalbumin has one Ca2+ binding site and several Zn2+ binding sites as well as many intermediary folding states, making it ideal for research involving the effects of cation binding on protein interactions as well as research on protein misfolding.1 α-Lactalbumin gives milk an antimicrobial property, making it important for infant health,…

proteins like NSF, SNAPs (soluble NSF attachment proteins), and SNAREs including SNAP25, VAMP and syntaxin. With these identified proteins, Rothman laboratory developed the SNARE hypothesis describing a model of SNAREs involvement in vesicle targeting and fusion. Their model explains that a transport vesicle (v-SNARE) and a target membrane (t-SNARE) with the aid of NSF, SNAPs and other yet unknown proteins bring the v-SNARE in close proximity to the t-SNARE facilitating membrane fusion (Sollner…

There are factors that can affect the enzymatic activity. Enzymes are known to speed up the biological reactions. It is specific to its substrate which in this experiment is known to be catechol. The catechol oxidase is used in all parts to express the concentration in the enzyme reacting into ortho-quinone (Schultz, 2006). The catechol oxidase if from the potato that was used in this experiment. The enzyme was extracted as the full strength after centrifugation and used the homogenization as…

Epigenetics, a contemporary and often misunderstood topic in the field of biology, was first defined in the early 1940s as “the branch of biology which studies the causal interactions between genes and their products with bring the phenotype into being” (Dupont 2009). Since then, as understanding of the human genome has developed and grown, a complex array of errors and dysfunctions in the processes of gene regulation, expression, and replication have been shown to cause, or correlate strongly…

Before we can talk about what a polymer is, we need to know about ATOMS and ELEMENTS and MOLECULES. To learn about atoms and elements and molecules, click here. Paul Lemur This is PAUL LEMUR. He's a lemur named Paul. Polymers are made up of many many molecules all strung together to form really long chains (and sometimes more complicated structures, too). What makes polymers so fun is that how they act depends on what kinds of molecules they're made up of and how they're put together. The…

(Faravelli, 2005). NSD1 contains 8088 base pairs, 23 exons, and translates into a protein that is 2696 amino acids long (Faravelli, 2005). This gene, along with the rest of the NSD family, have been found to be strongly linked to cell growth and differentiation (Faravelli, 2005).…