Buffer zone

that contains a corresponding component that binds with the sample at certain conditions. These conditions vary from acidity level, contending ligands, to ionic strength (Dean). Following the formation of the desired interaction, another component (a buffer) is used to wash unwanted elements that may also be in the column. The column…

taken from a cut and blended potato. The blended potato mixture was strained and put into a centrifuge, the liquid that was in the centrifuge contained the enzyme and remained on ice. The enzyme was placed in test tubes that had varying levels of pH buffers, substrate concentrations and temperatures; the pH, substrate concentration and temperature were the three variables. The data showed that each variable contained an optimum level at which the enzyme was most effective. Intro: Enzymes are…

acetate/acetic acid 10mM pH5 and 10 ml of protein extract containing bacteriocin were injected into this. After washing the column with sodium acetate/acetic acid 10 mM pH5 (Buffer A), elution was performed using a linear gradient from sodium acetate/acetic acid 10mM pH5 in 0 minutes to 100% of NaCl 1M in sodium acetate/acetic acid 10mM pH5 (Buffer B) in 25 minutes in ÄKTA pure chromatography system (GE Healthcare), with a flow rate 1 ml/min. Protein and peptides absorbance were detected at 280…

Throughout this acid, base and buffer lab, this will allow each individual to gain an enhanced sense of knowledge of how these different aqueous solutions work as a buffer system or how they are unable to perform as buffer system mixed with two solutions together. This can be identified through the colour change to measure pH behaviour in several aqueous solutions, therefore the different factors that will cause the change of pH. This will gain understanding of how this relates to the biological…

that certain organisms posed. Periwinkles dominated the region increasing interspecific competition within the microhabitat. Since the site was studied during high tide and was barely submerged, it indicated that perhaps the area was in the subtidal zone. Due to the barnacles’ constant need of submersion, this…

steep, and the sinuosity was meandering/braided. The embeddedness of the rocks in the stream was about 25%. There was quite an abundant amount of vegetation, the dominant plant species were conifer trees, deciduous trees, and herbaceous. The riparian zone was very abundant, there was a lot of moss, long grass, and shrubs. Now for animal life in the water, we saw many animals including fish, water bugs, crayfish, turtles, and even a leach. For animal life around the water we saw salamanders,…

PE Coursework Strengths The sport I have chosen is football. One of my main strengths (components of fitness) in football is my speed. The definition of speed is the ability to move quickly across the ground or moving limbs rapidly to grab or throw. In football, you use your speed for e.g; When you’re attacking and you are dribbling past a defender, you can use bursts of speed to blow past him. This means I get closer to the goal and have a better chance of scoring for me and my team. Also,…

It was a nice sunny afternoon. We had gotten the news that the weather wasn’t going to be good. Our bus ride was more calm than usual as I didn’t hear much talking. I guess we were a little excited we were going to play on turf. I personally didn’t really care about the turf but I was concerned, about the fact, we were going to play older players. Coach gave us a heads up to play our hardest. Only five minutes into the ride and I was getting the nerves I always got. It felt like someone was…

“habitable” planets have been found. The two most discussed are called KOI 326.01 and Kepler 438b. There are quotes around the word habitable because habitable doesn't necessarily mean perfectly livable. Habitable means that it is in the Goldilocks Zone, and has sufficient gravity. A habitable planet could be very small, partly gaseous,…

Speedball is a game we played by combining, three sports soccer, football, and basketball. The game starts by putting the ball on the middle of the football field. Both teams have to be in position on their own side of the football field or with the position of soccer ball The ball has to be kicked forward to start the game. Speedball is played by the position of the ball whether the ball is on the ground or a ball is in the air. When the ball is on the ground, it can only be played like a…