Buffer solution

velocity of the enzyme, as well as determining Km. For part three which was determining the nature of the active site, six additional cuvettes were prepared by adding water, 10 mM catechol, and 50 mM phosphate buffer for a total volume of 1.35ml represented below: Water (mL) Catechol (mL) Buffer (mL) 0.60 0.0 0.75 0.54 0.06 0.75 0.48 0.12 0.75 0.42 0.18 0.75 0.36 0.24…

standard methods (APHA,2005). A.DETERMINATION OF pH: The electrode of pH meter was calibrated with standard buffer solutions of pH 4, 7 and 9. The electrode was then dipped in the sample and its pH was noted down. B.DETERMINATION OF ELECTRICAL CONDUCTIVITY: Conductivity was measured by using soil and water analyzed kit. The electrode was conditioned with KCl and calibrates with 0.01M KCl solution. The sample…

will start to denature in room temperature, and actually work in high temperatures. I hypothesize is that any pH outside the range of 7-7.3 would denature the enzymes. My predictions is that the enzymes will denature in the most acidic solution, but in a basic solution it will…

microcentrifuge tube. 0 µL of the protein standard was added to Tubes 1a and 1b, 20 µL was added to tubes 2a and 2b, 40 µL was added to tube 3a and 3b, 60 µL to tubes 4a and 4b, 80 µL in tubes 5a and 5b, 100 µL in tubes 6a and 6b. 50 µL of the standard protein solution were added to each of the unknowns. Next various quantities of DI water were added to each tube. 1000 µL of DI water was added to tubes 1a and 1b, 980 µL was added to tubes 2a and 2b, 960 µL was added to tubes 3a and 3b, 940 µL…

What four elements make up 96% of all living matter? _________________________________________ Concept 2.2 An element’s properties depend on the structure of its atoms 2. Below is a model of an atom of helium, correctly label the electrons, protons, neutrons, and atomic nucleus. 3. What is the atomic number of helium? _________ Its atomic mass? ________ 4. Consider this entry in the periodic table for carbon. What is the atomic mass? ______ atomic number? _______ How many…

According to http://www.encyclopedia.com, DNA which stands for deoxyribonucleic acid is used for human genetic makeup. It has different sequences of bases and exist in human body. The sequence of it nucleotides are A, T, G, C; or, adenine, thymine, guanine, and cytosine, respectively. A DNA fingerprinting, is a DNA pattern that has a unique sequence such that it can be distinguished from the DNA patterns of other individual. The two major uses for the information is for personal identification…

and 10 standard buffer solutions are recorded. This reading are obtained using pH meter .The mv reading versus pH are plotted excel to generate a calibration curve, from the equation of the curve generated, the pH for each replicated sample and the analytic solution can be determined. Task 2. Standardization of HCL. The titrant solution of 0.02 m HCL and 0.08 M NaCl is standardized using a solution consists of a mixture of 0.05 M Na2CO3 and 0.085 M of NaCl as analytic solution. 50 ml the…

Phosphonates as inhibitors for Pks13-TE Hypothesis for the aim 1 of the proposed project is that the fluorinated α–Aminophosphonates as a covalent irreversible inhibitor for the Mtb Pks13-TE by scission of the P-C bond. Previous studies have shown the fluorinated aminophosphonates (FAPs) demonstrated serine esterases inhibition via scission of the P-C bond as shown in Figure 2.1.A 4. FAPs are known to irreversibly inhibit serine esterases such as acetylcholinesterase16 , neuropathy target…

Quavian Belton Biochemistry I Dr.Nicholas Panasik October 6, 2015 PCR Optimization Abstract A Polymerase Chain Reaction (PCR) is a reaction that is set to catalyze the amplification of a copy of a piece of DNA. The aim of this lab was to optimize a PCR reaction by finding out the optimum concentration of MgCl2 needed. Six reactions were set up four with varying concentration MgCl2 and two primer control groups. The volumes and concentrations of each component of the PCR…

3. RESULTS AND DISCUSSION 3.1. Method development and optimization The main criterion is to develop successful HPLC method for the determination of FVS and its degradation product. The developed method should be able to determine FVS and its degradation product in a single run. This method should be accurate, reproducible, robust, stability indicating, free of interference from degradation products and straight forward enough for routine use in quality control laboratory. Regulatory agencies…