Buffer solution

vitally important, the UF step concentrates the protein product into a smaller volume of buffer, while the DF step alters the conditions the protein product is sitting in i.e. Buffer exchange. These steps are required as they concentrate the protein product in smaller volumes of buffer allowing smaller equipment to be used, reduced time and resources and also ensures the protein product is in the correct buffer for the next step in…

With increasing pressure KM is nearly unchanged, indicating the binding volume, ΔVES≈0, in buffer solution, while kcat increases linearly, resulting a negative value of activation volume of reaction. The activation volume calculated from the slope of the curve in Figure 2d using equation 4.9 yields to -11±1 mL/mol that corresponds to a 2-fold reaction…

was used for SK-OV-3 and DU-145 cells, supplemented with 10% fetal bovine serum (FBS), and 1% penicillin−streptomycin solution (10,000 units of penicillin and 10 mg of streptomycin in 0.9% NaCl) in a humidified atmosphere of 5% CO2, 95% air at 37 °C. All bioassays were performed in…

Abstract Lantana camara is used as an ornamental plant which belongs to the family Verbenaceae. Is a small perennial shrub native to central and south America. Also known as big-sage, red-sage, tickberry. Is an invasive species. Present in many different colors depicting it’s maturity level. Can grow in variety of climatic conditions and soil. Is known to be toxic to livestock. Ingestion by grazing animals causes intrahepatic cholestasis and associated liver damage. Keywords: Lantana camara,…

‘Protein’ plays a vital role for our body as we cannot survive without it. Protein is an important building block of bones, muscles, cartilage, skin and blood. It is also a macronutrient, means the body needs relatively large amount of it. Recently, almost everyone starting from the teenage ageto the age of 30-40 wants to stay healthy . There are bodybuilders too who are working hard to accomplish their goal, they ingest protein shakes. Since protein shakes fulfill the purpose of deliverying the…

limiting enzyme in the production of glucose (Wei et al., 2012). In this experiment the substrate for cellobiase, cellobiose, was replaced with gluco p-nitrophenyl. Gluco p-nitrophenyl turns yellow when it is exposed to the stop solution. The absorbance of the solution that contains the Gluco p-nitrophenyl can be measured using a spectrometer. The purpose of the experiment conducted was to compare the rate of…

and placing them inside of a syringe with sodium bicarbonate buffer. We then pulled the plunger part of the syringe back which lets the bicarbonate solution infiltrate the air spaces in the piece of leaf. After the air spaces are completely infiltrated and the leaf discs sank to the bottom of the syringe we then placed the discs and the solution inside of that syringe into the beaker that was holding another 40 ml of bicarbonate solution. Then to test the actual…

The experiment performed provided insight on the importance of acid-base interactions and the pH of solutions that are involved in a variety of aspects including industrial, environmental, and biological contexts. Back titration was used to determine the number of moles of HCl acid that were neutralized by a single tablet of several variations of commercial antacids. In theory, the antacid that neutralized the largest number of moles of acid was found to be the most effective. The results…

Before working with a chromatography column to separate or isolate specific molecules, it requires understanding the theory behind the technique. Most of chromatography works based on the same general principle: a sample mixture in a mobile phase (buffer) passes over a selective matrix that called stationary phase. The stationary phase or the matrix inside the column is a composed cross-linked polymer bead with engineered pores or cavities (#2 Lehninger, 87). In a well-packed column, the large…

It is suggested that future studies should consider the amount of water used to dilute the buffer when conducting electrophoresis, as that might affect the migration ability of the DNA. The experimental design should also consider the amount of restriction enzymes, and the amount of DNA used, if more fragments of the DNA are able to be cut, then…