Buffer solution

Purifying DNA to Estimate its Purity using PCR Amplification of VNTR to Load and Run Agarose Gel Introduction The study of this experiment was the Dopamine transporter gene. This gene is associated with different brain disorders like bipolar, as well as certain behavioural traits such as ADHD.[1] Dopamine transporter gene is a presynaptic plasma protein containing different VNTRs in it’s UTR and plays an important role in restricting the activity of dopamine by rapid reuptake into the…

The activity changes as measures for the absorbance and temperatures shown in Figure 1 below. Each cuvette contains the same solution, but all were tested at different temperatures measured in Celsius. Cuvette 1 is labeled as 0 Degrees Celsius which is shown below in Figure 1. The overall change in absorbance was higher than the room temperature controlled solution. Cuvette 2 is labeled as 23 Degrees Celsius and is shown below in Figure 1. This substance was used as the control throughout the…

reaction was the bean extract. To mimic the FAD that is used in the Krebs cycle, we used 2,6-dichlorophenolindophenol (DPIP). When DPIP is chemically reduced (like the FAD would be), it changes color from blue to clear. The carrier was the phosphate buffer. The inhibitor that was used to test/slow the reaction was Malonate and the substrate used was succinate. In order to view the rate of metabolism that was occurring, we used a spectrophotometer to measure the color absorbance. When DPIP is…

A chemical reaction is the change of a molecule from one arrangement to another. Our whole bodies are made up of cells, which are chemical systems. To sustain life there must be chemical reactions all throughout the body (Barnhart and Hopper 2011). At what rate the reaction can occur is dependent on the activation energy. Activation energy is the amount of energy the reactant molecules must surpass for the reaction to occur (Mathew Bui et al. 2006). Temperature and catalysts are the most basic…

With this experiment intend to prove Salem Lakes ability to buffer chemicals used in the water treatment process versus that of the Yadkin River. Using a jar test machine, simulated the addition of an acid in the form of Al2SO4, and a base NaOH to the coagulation process. Salem Lake does show a preferred buffering at higher acid doses, though Yadkin River is more efficient at the low to mid-range dose. INTRODUCTION There is a distinct difference in the buffering capacity of Salem Lake versus…

Error bars represent SEM Experiment Catalase Treatment t-test matchup p-value Effect of varying substrate concentration Regular Catalase (phosphate buffer, pH 6.8) 0% vs 0.1% 0.1% vs 0.2% 0.2% vs 0.4% 0.4% vs 0.8% p= 0.002518333 p= 0.000243814 p= 1.35676E-05 p= 0.000258456 Table 1 – Statistics table for average reaction rate for varying substrate concentrations In the second part of the experiment…

large online databases.

When you get to the section where the animation separates into plant and animal cells, select animal cells: 
2. What are the two steps taken at the beginning of the process to break down the cell membrane? a. Adding lysis solution to tube which dissolves membrane bound organelles b. Twisting a clean pestle against surface and grinding the tissue which breaks up the cell walls and other tough materials 3. What is the first step to separate the cellular debris from the…

In this experiment, three strains of tetracycline resistant E. Coli were tested. First, the bacteria were diluted via serial dilution in order to more accurately count and quantify the bacteria. To ensure that the three different strains of bacteria are not cross contaminated, label everything either Farm A, Farm B, or Farm C depending on what is being tested. There are six agar plates for each farm, three of the plates are tetracycline plates (which are labeled) and three are unlabeled. For…

Name this solution as solution W so there is less confusion later on during the procedure. Next, obtain 3 ml of cyclohexanone and 2.5 ml of 2-furaldehyde in 15 ml 95% ethanol. Name this solution E for less confusion later on during the procedure. Once this step is complete, 25 ml of the solution W and 5 ml of the solution E needs to be cooled in an ice-bath, but make sure that the 5 ml and the 25 ml are separated and not added together while being cooled. Once both of the solutions are cooled,…

pylori product solutions were diluted to various concentrations. Each H. pylori product was assessed over eight concentrations. Samples were diluted in two-fold serial dilution steps starting with 100 µg dry weight per well. H. pylori product samples were prepared in PBS. LPS solution was made at a concentration of 2µg/mL in RPMI-2HS. Assay was composed of negative controls, positive controls, and…