Buffer overflow

Abstract The main goal of the buffer capacity experiment was to analyze how the addition of acid or base and dilution affected the measured pH. The results showed that when the buffer systems were under acidic conditions their pH values decreased and increased when exposed to a basic environment. The main goal of the ionization of the amino acid experiment was to titrate an unknown amino acid with a strong base in order to produce a titration curve. The titration curve was used to evaluate the…

3.1: Ligand preparation: A series of benzimidazole derivatives were used to prepare the ligand dataset using benzimidazole as the query molecule. All the inhibitors selected comply with the Lipinski rule of five. A total of 50 molecules were taken up for the study. 3.2 Virtual screening: To further screen the compounds, additional similarity metrics were calculated by ROCS. ROCS is a shape based superposition method used to perceive similarity between molecules based on their three dimensional…

1. List the steps you would take if you were to break a beaker with a pH 9 solution. First, once a spill occurs, ensure everyone (especially the lab instructor/advisor) around the area or in the lab knows about the occurrence. Second, it would be best to make sure that you have not been contaminated with the chemical, meaning that you should take care of yourself by washing the affected areas. Third, once your hands are dry, you should then make an effort to neutralize the solution of pH 9 as…

Metronidazole Systematic (IUPAC) name: 2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethanol Bioavailability : 80% (oral), 60-80% (rectal), 20-25% (vaginal)122,123 Protein binding : 20% 122,123 Metabolism : Hepatic 122,123 Half-life : 8 hours 122,123 Excretion : Urine (77%), faeces (14%) 122,123 Formula : C6H9N3O3 Mol. mass : 171.15 g/mol Melt. point : 159–163 °C (318–325 °F) Metronidazole is a nitroimidazoleantibiotic medication used particularly for anaerobic bacteria and protozoa.…

RESULTS AND DISCUSSION 3.1 Effect of pH In the pH range 4.0 to 8.5 the rate of degradation of Basic fuchsin was investigated. The results are reported in Table 1. Table 1: Effect of pH [Basic fuchsin] = 0.8 × 10-5 M Light intensity = 60.0 mW cm-2 CuO/Al2O3 = 0.12 g pH Rate constant (k) × 105 (sec-1) 4.0…

deionized water was used to plot a standard curve, the activity of pectin was determined relative to 1mM of the standard. The stock solutions include 300mM acetate buffer, pH 3.6, 10mM TPTZ (2, 4, 6-tripyridyl-S-triazine) solution in 40 mM HCl and 20 mM Fecl3.6H2O. The working solution was prepared fresh by mixing 25 ml acetate buffer, 2.5 ml TPTZ and 2.5 ml FeCl3.6H2O, and its temperature raised to 37ºC before use. 150µL of pectin solution was mixed with 2850µL of FRAP solution for 30 min in…

one of these famous wolves. But recently, the wolf population in Denali National Park has been surrounded by controversy. Denali National Park along with members of the public have been fighting the Alaska Board of Game to establish a hunting free buffer zone along park boundaries in effort to maintain the wolf population that has recently began to fall.…

Proteins are known as the building blocks of cells in all living organisms. Many hormones are made up of proteins and the digestive system, immune system and blood replies on proteins. Therefore, protein is an essential part of healthy diet, and integral to development of the body. The purpose of performing this lab is to analyzing the activity of TEM-1 B-lactamase and how it reacts with high temperatures, chaotropic salts, detergents and pH values. INTRODUCTION: Primary protein structure is…

that contains a corresponding component that binds with the sample at certain conditions. These conditions vary from acidity level, contending ligands, to ionic strength (Dean). Following the formation of the desired interaction, another component (a buffer) is used to wash unwanted elements that may also be in the column. The column…

taken from a cut and blended potato. The blended potato mixture was strained and put into a centrifuge, the liquid that was in the centrifuge contained the enzyme and remained on ice. The enzyme was placed in test tubes that had varying levels of pH buffers, substrate concentrations and temperatures; the pH, substrate concentration and temperature were the three variables. The data showed that each variable contained an optimum level at which the enzyme was most effective. Intro: Enzymes are…