Bradford protein assay

The synthesis of Aa-g-poly(MAA) involve the optimization of various reaction conditions like initiator concentration, monomer concentration, crosslinker concentration, solvent concentration and pH. After the optimization of various reaction parameters the replications were carried-out in triplicates for the reproducibility of the results and statistical of the results obtained. It was performed using the Statistical Package for Social Science (SPSS) version 10. Statistical results of the…

Skim milk delivers the high-quality protein you need for stronger bones and bigger guns. ARE CALORIES FROM FAT, PROTEIN, AND CARBS EQUAL? Scientists study how your body processes calories from different food sources. Which combo is the best for weight loss? Goat milk? If you're trying to put on muscle, you better. Whole milk was once a staple for guys who were packing away calories and moving massive amounts of iron in order to go from pencil neck to bull neck. These days we know enough to…

the endosomes, kinases and phosphatases produces and hydrolyses specific phosphoinositides which identify endocytic membranes and allow them to recruit proteins from the cytosol that are involved in vesicle trafficking and other aspects of cellular homeostasis. Lipid mediators of signaling and recognition processes work through specific protein–lipid interactions, the levels of these signaling molecules are extremely small compared with the levels of bulk membrane…

1 enzyme Enzymes are biological catalysts. A protein compound Energy can reduce the carbon reaction. Enzymes to accelerate specific types of reactions. And the kind of substance that reacts. 2.1.1 catalysis of enzymes E is a catalyst (enzyme). S is a precursor called chop, nitrates and substance P is a product. E + S ---------------> E S ---------------> E + P. Complexometry 2.1.2 Factors affecting the enzyme. 1) What type of controlled…

Omega-3 Fatty Acids Omega-3 fatty acids play a big role in a number of bodily functions. They are a group of polyunsaturated fatty acids and are usually found in fatty fish such as salmon, tuna and trout in the form of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) (NIH, 2017). Another type of omega-3, called alpha-linolenic acid, is found mainly in plants. Omega-3 fatty acids play a role in muscle activity, blood clotting, digestion, fertility, cell division and growth, brain…

ACR response curve for both groups Graph9: DAS28 response curves for both groups CASE STUDY 4: RITUXIMAB & ITS BIOSIMILAR Rituximab is monoclonal antibody used to reduce auto-immune disorders, transplant rejections, etc. Rituximab binds to CD20 proteins which are found on the surface of B cells and decrease the effectiveness of natural killer cells in killing these B cells. It destroys B cells and hence treat dysfunctional or excessive number of B cells. Rituximab was developed at IDEC…

FTIR analysis of the stem bark extracts of Cassia fistula has absorption bands and the wave numbers (cm-1) of the prominent peaks obtained were described in Table 2. The peak at a frequency of 3463 cm-1, 1618 cm-1, 1448.3 cm-1 and 1241.9 cm-1 were strong while the others vary from medium to weak. Discussion: Phytochemical studies revealed the presence of secondary metabolites like tannins, flavonoid, carbohydrate, saponins, glycosides and phenol in the leaf extract while tannins, flavonoids,…

biologically relevant molecules such as proteins, nucleic acids, carbohydrates, and lipids play a key role in signal transduction. Thus, the relative alignment of the two interacting proteins can affect the type of signal produced. Therefore, docking is used for predicting (assuming) both the strength and the type of signal produced. Molecular docking is one of the most frequently used methods in structure-based…

that is responsible for destruction of toxic H2O2 released during certain metabolic processes.The activity of catalase can be determined by measuring the decrease in H2O2 concentration which is the substrate. Introduction: An enzyme is a large protein that acts as a biological catalyst which changes the rate of a reaction. It provides an active site which is an environment where a reaction can take place this is made up of amino acids. The structure and shape of the substrate, the structure…

Laccase: Dynamic site structure and reactant cycle. (a) Model of the catalytic cluster of the laccase from Trametes versicolor made of four copper molecules. Type I (T1) copper presents the trademark blue colouring to the protein and is the site where substrate oxidation occurs. Type 2 (T2) and Type 3 (T3) copper form a trinuclear center, where the reduction of molecular oxygen and release of water takes place. (b) Schematic portrayal of a laccase reaction cycle producing…