Bradford protein assay

co-immunoprecipitation of HEK293T cells stably expressing FLAG-RagB who are starved from 50mins and then re-stimulated with glutamine for 10mins and immunoblot for endogenous FLCN levels. If FLCN is glutamine sensitive, you should see a transition from protein interaction between FLCN and RagB seen under…

wheat kernels in 150 μm sieve; Wwheat in 75μm sieve = weight of wheat kernels in 75 189 μm sieve; Wpan = 190 weight of empty pan; and Wmaize meal = weight of maize flour. 191 Determination of Crude Protein 192 The crude protein content of the roasted maize was determined in duplicate by Dumas combustion 193 method as described in AACCI Approved Method 46–30.01 (AACC, 1999) using a Leco, model 194 FP-528 (Leco Africa, Kempton Park, South Africa). Calibration of the instrument was done using 195…

high in saturated fat like cakes, pastries, pies, processed meats, commercial burgers/pizza, fried foodstuffs, potato chips, crisps, biscuits and other savory snacks. One unit weight of fat contains twice the kilojoules (energy) of carbohydrate or protein. Thus, if we consume foods and drinks with too much dietary fat, it can be difficult for us to maintain a healthy body weight. All through the day, we consume a wide range of regular, healthy foods. This by itself can give us easily a small…

DNA ISOLATION INTRODUCTION: Deoxyribonucleic acid (DNA) isolation is an extraction process of DNA from various sources. Methods used to isolate DNA are dependent on the source, age, and size of the sample. Despite the wide variety of methods used, there are some similarities among them. In general, they aim to separate DNA present in the nucleus of the cell from other cellular components. Isolation of DNA is needed for genetic analysis, which is used for scientific, medical, or forensic purposes…

5.3. Results of the 3rd experiment. (Co-digestion of kitchen waste with cow dung slurry). 5.3.1. Results for the physicochemical characteristics Sl. No Parameter Fresh cow dung slurry Fresh food waste slurry Fresh cow-dung + fresh food waste slurry Digested slurry 1 pH 6.4 6.0 6.2 7.0 2 Total solids (%) 8.5 9.1 8.8 6.16 3 Moisture Content (%) 92 90.5 91.25 94.33 4 Volatile Solids (%) 92.9 94.5 93.7 82.50 5 Percent carbon 2.27 3.03 2.65 0.27 6 Phosphate(mg/100g) 0.24 0.310 0.275 1.010…

PCR (Polymerase chain reaction) Polymerase chain reaction (PCR) is a laboratory method that is used to form number of copies of a specific region of DNA interested in. The principle of the polymerase chain reaction is based on the natural replication process of DNA and complementary nucleic acid hybridization. The goal of the PCR is to produce required amount of the target DNA region that is be analyzed. PCR amplified DNA is sent for sequencing and visualized by gel electrophoresis. It also can…

FDE 310 Food Materials Laboratory Report Determination of Reducing Sugar Content INTRODUCTION The objectives of this experiment is to determine reducing sugar content of food sample by performing Fehling method and to identify invert sugar content of sugars by using polarimetry and also to learn property of invert and reducing sugar. Carbohydrases represent a large class…

Consequences of Antigen-antibody Binding:- Interaction of antigen and antibody is a bimolecular association and it never lead to an irreversible chemical modification in either the antibody or antigen. The association between antigen and antibody includes various non-covalent interactions between the antigenic determining factor (epitope) of the antigen and the…

CHAPTER 1 INTRODUCTION Since time immemorial microorganisms have played a significant role in the life of mankind owing to the production of the myriad enzymes and secondary compounds that they synthesize (Demain 1981). These micro organism live in a highly diverse environment ranging from hot springs to deep ocean sediments. In the hyper diverse environment where one can find a specialized and unique biological niche is the intracellular space between cells of higher plants acts as suite and…

In order to evaluate the efficiency of RAPD-PCR fingerprinting for Cajanus cajan L. cultivar, the DNA templates for 7 RAPD primers banding modality were used. The total number of magnified fragments in the Cajanus cajan L. cultivar value of each primer was represented in Table 2. Total of 18 bands were obtained for all primers. The size of the amplified bands was ranged between 1000 bp and 300 bp. Primers OP-A5, OP-B4, OP-B9, OP-C5 and OP-E15 showed at least three specific fragments. Primer…