Bradford protein assay

modification and secretory expression like in higher eukaryotes. An increasing number of yeast strains are available for protein expression that are said to increase the yield of the protein, improves the affinity tagging of the proteins, reduces protein breakdown, allows the addition of non-native amino acids (Selenomethionine) into the protein and defines the composition of N-glycan in the protein produced. Following are the existing genera of yeast…

as common. Finally, amino acid sequences of proteins…

They are made within the nucleolus from 60% RNA and 40% protein, and consist of two subunits, one large and one small. When produced, the subunits stay separate to exit through the nuclear pores: it is only because of their miniscule size that they can fit through. Many ribosomes then bind themselves to the RER, although some stay free in the cytoplasm. Free and bound ribosomes both create polypeptide chains, which are used to make proteins. To create a polypeptide chain the large and small…

DNA known as protospacers indicative of particular bacteriophages encountered by bacteria. These sequences are then used as a template for RNAs capable of recognizing viral DNA within a cell and as a means to bind the endonuclease CRISPR associated protein 9 (Cas9). These complexes allow the enzyme to be shuttled to the target DNA to perform a cut on one or both strands of the foreign DNA in either of the enzyme’s two active sites. Recently, scientists have repurposed this bacterial defense…

Cellular Respiration takes place on the level of the cell, inside the mitochondria. This is the process of breaking down food in the presence of oxygen to form ATP. ATP stands for Adenosine Triphosphate which is an energy bearing molecule found in all living cells1. Things such as bacteria can perform this with their outer membranes. When a person runs, they use cellular respiration to create energy in the form of ATP which allows their muscles to move. Many other energy-consuming reactions of…

Corporation 's have lied to you about consumers and minimum wage workers being the backbone to their industry. Enzymes are. Enzymes are biological protein catalysts that encourage chemical reactions by lowering the activation energy needed for the reaction to take place. Once the reaction is complete, enzymes are left unchanged and able to move on to the next reaction. For this reason, enzymes have proven to be valuable resources for industries. The reusability of enzymes are especially useful…

Proteins are large, complex molecules found throughout the body. They are polymers made out of different types of small unites called amino acids. Each amino acid consists of a central carbon atom, which is attached to a hydrogen atom, an amino group, a carboxyl group, and a unique side chain or R group. There are twenty different types of amino acids and each one of them has different chemical properties. Amino acids are held together by covalent peptide bonds therefore the proteins, chains of…

Fingernails are capable of showing sicknesses or diseases that are happening inside the human body. The plate of the nail is made of a protein named “keratin” which is transparent and flexible but durable enough to retain a shape and protect the nail bed. Underneath the nail plate is the nail bed which is composed of two parts, the dermis and the epidermis. The purpose of the nail bed is to house the nail matrix, which grows the nail and stabilize the nail’s foundation. The nail plate and nail…

The question my group tried to answer was “Which protein solution contained protein, starch, and sugar, and if so how much?” The protein supplements we had were supplments 4 (Kroger Whey & Soy Protein- Vanilla),6 (Vega Protein and Greens) ,and 8 (Pure Protein Natural Whey Protein). We also had 3 reagents (Biuret’s, Lugol’s ,and Benedict’s). We had two different experiments, the first experiment was to get qualitative data. The second was to get quantitative date. The first experiment we tested…

Movement of cells are important since some cells depends on movement to carry out its major function. For example, sperm cells need to move from one place to another place to carry out reproduction. Movement of cells are supported by cytoskeleton system of the cells. The cyctoskeleton system are composed of microtubules, microfilaments and intermediate filaments. However, for unicellular organisms such as Paramecium, the movement of cells are regulated through cilia or flagella. Cilia can be…