Bradford protein assay

Daniels, L.B., Glew, R.H., Diven, W.F., Lee, R.E., and Radin, N.S. (1981) An improved fluorometric leukocyte β-glucosidase assay for Gaucher’s disease. Clin. Chim. Acta, 115 (3), 369–375. 13. Wenger, D.A., Clark, C., Sattler, M., and Wharton, C. (1978) Synthetic substrate \s s-glucosidase activity in leukocytes: A reproducible method for the identification of patients and carriers…

Protein-protein interaction can be defined as the interactions between different protein molecules within the cellular organization leading to formation of large protein complex which is modulated by non-covalent interactions such as hydrophobic interaction, hydrogen bond, van der Waals interactions etc. Protein-protein interaction plays an important role in functioning of body’s significant mechanism such as replication, transcription, translation, signal transduction, cell cycle, etc. Thus,…

Aleem Mohammad Stone Bio 2107 Lab 14 Sept. 2015 Accuracy Check of Protein Concentration vs FDA Labels Abstract The issue of food label accuracy is becoming very popular in the United States. The widespread awareness of genetically modified organisms and greater insight about the mass production of food lead to many questions and uncertainty about the labeling of edible products. The US Food and Drug Administration (FDA)’s accuracy in labeling will be tested in this experiment to see whether or…

three-dimensional structure of a protein not only defines its shape and size, but also its function. Proteins consist of a long chain of 20 amino acids folded up into complex shapes. Each nonpolar amino acid folds into the interior of the proteins during the folding stage, as they are hydrophobic. The primary and secondary structure determines the hydrophobicity of a protein. Each nonpolar amino acid folds into the interior of the proteins during the folding stage. For instance, membrane…

A relationship with numbers and codes is my fate. I learned math for an Olympic Mathematics Competition that I’ve been involved in since I was 9 years old. I became familiar with algorithms like congruence, Horner algorithm and graph theory before I could use a computer. Now as a senior in the school of mathematics at XX University, one of the top three mathematical schools in China. I chose math as my major to build a good mathematical foundation to thoroughly learn about computer science. With…

material - DNA that makes up the genes and chromosome. The Ribosome: ~Made of dozens of different proteins as well as RNA, also found in large numbers in the cytoplasm of living cells. Ribosome are typically composed of two subunits: • Large subunit • Small subunit “ Free” Ribosome makes proteins that remain inside the cell, “Bound’’ Ribosome make proteins that are exported from the cell. ~Protein Synthesis ~Chemical reactions is molecules that are brought into contact with one another.…

Alkaline phosphatase (AP) is a homodimeric enzyme complex that is commonly found in a wide range of organisms, from bacteria to all tissues of the human body. AP is a zinc metalloenzyme (1), in which metal ions play a key role in the regulation of catalytic activities and stabilization of enzyme-substrate complex. As proposed by Gettins and Coleman using NMR studies (10), each active site of AP comprises of three metal binding sites, which acknowledged as M1, M2, and M3. Two zinc ions bind to…

increases the ranges of non-canonical amino acids. It is shown that misacetylationi in polypeptides show an increase in specific disease. Site-specific incorporation of genetic code reprogramming have demonstrated powerful techniques for investigation proteins and as a tool for biologically-mediated organic chemical synthesis of peptides. The replacement of amino acids with the non-canonical amino acid allows scientist to understand the role of a specific amino acid in the polypeptide molecules.…

Due to limited time constraints and having lab only once a week this lab was broken up into five periods, the last one specifically to go over the results. Day one consisted of extraction of plasmid DNA, which we used Resuspension Buffer to break up our pellet after removing the supernatant (liquid) after centrifuging our overnight culture. Then Lysis Buffer was used to break down the membrane of the cells so that we could then add Neutralization Buffer to bring our sample from basic to neutral…

molecular mechanism of invasion, survival and pathogenesis. F.alocis ATCC 35896 and D-62D strain have variation in their protein profile. F.alocis genome has a total of 15 different proteases. These proteases may be membrane bound or extracellular. Among membrane bound proteases CaaX proteases are present in both the strains. In S.gordonii these proteases are known to play a role in protein transportion and protection of bacteria from bacteriocins. Protease HMPREF0389_00122 is present in the…