Bradford protein assay

There are factors that can affect the enzymatic activity. Enzymes are known to speed up the biological reactions. It is specific to its substrate which in this experiment is known to be catechol. The catechol oxidase is used in all parts to express the concentration in the enzyme reacting into ortho-quinone (Schultz, 2006). The catechol oxidase if from the potato that was used in this experiment. The enzyme was extracted as the full strength after centrifugation and used the homogenization as…

Epigenetics, a contemporary and often misunderstood topic in the field of biology, was first defined in the early 1940s as “the branch of biology which studies the causal interactions between genes and their products with bring the phenotype into being” (Dupont 2009). Since then, as understanding of the human genome has developed and grown, a complex array of errors and dysfunctions in the processes of gene regulation, expression, and replication have been shown to cause, or correlate strongly…

Before we can talk about what a polymer is, we need to know about ATOMS and ELEMENTS and MOLECULES. To learn about atoms and elements and molecules, click here. Paul Lemur This is PAUL LEMUR. He's a lemur named Paul. Polymers are made up of many many molecules all strung together to form really long chains (and sometimes more complicated structures, too). What makes polymers so fun is that how they act depends on what kinds of molecules they're made up of and how they're put together. The…

(Faravelli, 2005). NSD1 contains 8088 base pairs, 23 exons, and translates into a protein that is 2696 amino acids long (Faravelli, 2005). This gene, along with the rest of the NSD family, have been found to be strongly linked to cell growth and differentiation (Faravelli, 2005).…

Maple Syrup Urine Disease (MSUD) is a rare, autosomal recessive metabolic disorder in which the body is unable to process certain amino acids properly. It causes brain damage and progressive nervous system degeneration. MSUD is caused by mutations in three different genes: BCKDHA, BCKDHB and DBT (Warrell, Cox and Firth, 2012). The BCKDHA gene causes MSUD Type Ia due to the mutation in the E1α subunit, BCKDHB causes Type Ib due to the mutation in the E1ᵦ subunit, and DBT causes Type II due to…

Benzimidazole and its derivatives were synthesized according to the reported literature (Wagner EC et al., 1943). The compounds are benzimidazole ;1-(4-((1H-benzimidazol-1-yl) methylamino) phenyl) ethanone; 2-butyl-1H-benzimidazole; 2-methyl-1H-benzimidazole. The stock solutions are prepared by dissolving the compound in DMSO. Antimicrobial activity was tested by the disc-diffusion method under standard conditions using Mueller-Hinton Agar (MHA) and the antibiotics used as listed below, Table…

This essay provides a brief description of Familial Adenomatous Polyposis (FAP), a mutation on the APC gene (Cleveland Clinic, 2016, pg. 1). It discusses the beginning ages for FAP and some genetic testing options for said disease, including how accurate such tests are. The remaining portion of this paper explores my stated opinion on whether or not adolescents should be tested for late-onset genetic diseases, such as FAP. A Brief Description of Familial Adenomatous Polyposis (FAP)…

In this investigation, electrolyte content will be measured and compared to the sugar content to give a measurement of how drinks compare in terms of benefit during exercise. The independent variable will be the drink being tested, and the dependent variable will be the electrolyte and sugar content of that drink. Electrolytes are positively or negatively charged ions that are essential for survival. Positively charged electrolytes move towards a cathode, while negatively charged electrolytes…

(EPOCH (chemotherapy), 2015). This is often combined with rituximab which can be called R-EPOCH. Rituximab is a chimeric monoclonal antibody against the protein CD20 which is mostly found on the surface of immune system B cells. (Rituximab, 2015) Prognosis of Burkitt lymphoma (BL) in children, depends on the bulk of the disease at the time of diagnosis, Intensive systematic chemotherapy is the ideal choice…

Previously we were taught that our DNA is what make us unique. But what if I told you that our DNA didn’t do all the work? There is a protein in our bodies that makes our DNA coding complete and it’s called an epigenome. There are two proteins that make up one protein of a epigenome and they are histones. They are wrapped around the DNA and the Methyl group that are attached to it. Of course we are taught that our DNA makes up who we are as far as characteristics; However, epigenetics explain…