To determine optimal pH value for the hydrolysis of p-NPG by leukocyte GCase, we examined effects of variable pH (4.0-5.5) at a constant substrate concentration (5mM). Figure 1 shows the pH activity curves of leukocyte GCase assayed in the presence and absence of 6 mg per ml Sodium taurocholate. In the absence of the pure sodium taurocholate, the activity in the range of pH 4.0-4.8 was considerably lower. However, in the presence of pure Sodium taurocholate, leukocyte GCase was optimally active…
This experiment exemplifies the effects of the concentration of hydrogen on the strength of bone. As bone is majorly composed of calcium, and knowing that acids want to neutralize. Throughout the experiment, results show that the strength of the bone was compromised in acidic situations, but rather unaffected in situations where the concentration of hydrogen was basic. Even more noticeable than the change in pure tensile strength is the change in the maximum deflection before fracture.…
Abstract The main goal of the buffer capacity experiment was to analyze how the addition of acid or base and dilution affected the measured pH. The results showed that when the buffer systems were under acidic conditions their pH values decreased and increased when exposed to a basic environment. The main goal of the ionization of the amino acid experiment was to titrate an unknown amino acid with a strong base in order to produce a titration curve. The titration curve was used to evaluate the…
Bromination of trans-cinnamic acid Purpose and Introduction The halogenation of of trans-cinnamic acid using pyridinium tribromide was connected in the organic solvent glacial acetic acid in order to verify and test which addition mechanism the reaction would follow. In the anti-addition mechanism, bromines attach themselves on opposite sides of the alkene functional group in a compound. In a syn-addition, the attach to the same side of the alkene. The reaction synthesis was completed by using a…
The values that arise from the feed analysis were: the dry matter, crude protein, acid detergent fibre, neutral detergent fibre, pH level and ash. Specifically, the value for the dry matter is the corrected value in order to allow for acids, volatile acids and other components that lost on oven drying. The crude protein which is determined from the total nitrogen of silage is 88.33 (g/kg DM). The value of the acid detergent fibre which is a measurement of lingin, cellulose and lignified nitrogen…
At 20 seconds, pH = 7.35 2. At 40 seconds, pH = 7.29 3. At 60 seconds, pH = 7.24 4. Did the pH level of the blood change at all during this run? If so, how? Yes, the pH level of the blood decreased. 5. Was the pH level always within the “normal” range for the human body? If not, when was the pH value outside of the normal range, and what acid/base imbalance did this pH value indicate? No, acidosis occurred at 40 seconds when the pH dropped out of “normal” range to…
Plant Science - Seed germination and growth The research question: What is the effect of changing the pH of the soil on the germination and growth of mustard peas? Hypothesis: The greatest number of peas will germinate and will grow the most when the pH is 5 or 7. Theory: Enzymes play a vital role in the cells of plants (especially when growing) and enzymes work best when at their optimum pH value. Moreover, all chemical reactions have an optimum pH at which they work best (Howard and…
Part A: Background Information Acids and bases are very common in today’s society, and they can be found in most households. Acids can be found as by-products of air pollution, concrete cleaner, food flavouring and is produced by the muscle. Bases are found in most cleaning products as ammonia or sodium hydroxide, and they also treat indigestion. There are many ways that acids and bases can be defined by their chemical properties. The Bronsted-Lowry definition states that acids are proton…
Title- Experiment #9- Acid Base Titrations II: Potentiometric and Indicator Titrations Introduction- In this experiment, the reactions of three different acids with a strong base are studied by observing how the pH of the solution changes as the titrant is added. A plot of pH versus amount of titrant added will be constructed. Such a curve is called a pH titration curve. The equivalence point of the reaction will be determined from an analysis of the pH titration curve, and by the use of…
reaction. Determine the rate law of a particular reaction experimentally. Observe the effect of catalyst on the reaction rate. Examine the influence of temperature on the rate of this reaction. Calculate activation energy for the reaction from rate constants at two different temperatures. Practice the use of volumetric glassware. Master calculations of solution concentrations. Introduction: The following primary reaction will be investigated in this experiment: S2O8 2– + 2I– I2 +…