Bla Bla Bla

accomplished when a sexy local is by your side, directing your outing toward laid-back locales, not teeming with tourists. Spend a sexy afternoon with a woman in the artsy district of Cusco, Barrio de San Blas. Here, local artisans’ works are featured in a myriad of craft shops and galleries. The Barrio de San Blas neighborhood is one of the most picturesque in Cusco, and a delightful area to share a lazy afternoon becoming acquainted with a new…

Transformation is the process by which competent bacteria take up genetic material (in the form of naked DNA) from the environment. In this lab, the process of transformation was conducted by using a plasmid containing various genes called pGLO along with the bacterium Escherichia coli. In order for transformation to occur in Escherichia coli, repeated washes in the presence of CaCl₂ followed by heat shock treatment (42°C for 50 seconds) occurred to induce the competence of the bacterium.…

SA2 Expected outcomes and potential difficulties. The results of the experiments proposed in SA2 are expected to determine if POMC and AgRP neurons form synapses with MC3R VTA neurons, determine the identity of MC3R-expressing VTA neurons, and to map afferent and efferent projections of VTA MC3R neurons. GABA neurons are the second most numerous neuronal population in the VTA. Therefore, it is expected that a large proportion of TH negative MC3R-expressing VTA neurons will colocalize with GAD67…

The bla gene codes for beta lactamase which promotes antibiotic resistance for drugs classified as being a part of the penicillin family. In the pGLO plasmid there is a unique gene regulation system which controls the transcription of the green fluorescent…

When Does the Regulation of Vaccinations Go Too Far? When talking about vaccinations, the morality of individuals is one factor taken into consideration. There has been controversy regarding vaccinations causing autism, whether natural immunity is better than vaccine acquired immunity, why vaccines aren’t 100% effective, and what effects religious views have on the distribution of vaccinations. These questions tend to have difficult answers or none at all because they are based on the ethics of…

There are different forms of euthanasia; voluntary and non-voluntary. Both of which are considered to be illegal within the Criminal Code of Canada. Non-voluntary euthanasia is killing an individual who does not have the mental ability to consent to their own death. These types of people are individuals who are either in a vegetative state or are mentally disabled. The controversial debate ‘should non-voluntary euthanasia be legal?’ This question arises in the case of R. v. Latimer. Robert…

and Malcom X were major figures in developing Assata as a rebel much like they were for many blacks. However, Huey Newton the leader of the BLA was also a major factor that drove Assata to become a radical figure. The US government and white supremacists were arguably the biggest reasons Assata became a radical figure. This is because the US government and white supremacists were the ones oppressing…

simple structure and are quick to reproduce (Centers for Disease Control and Prevention, 2015). Because of this, scientists know a lot about E. coli. In this experiment, the E. coli will be exposed to a pGLO plasmid; each plasmid has an Ori, pBAD, araC, bla, and GFP. The Ori, origin of replication, is the site of DNA replication in the plasmid. On these DNA strands is the pBAD promoter, a sequence of DNA used to start the transcription of the GFP (green fluorescent protein), which only…

The Book of Kells is considered to be one of Ireland's most valuable national treasures as well as the finest illuminated manuscript created in early medieval Europe.Illustrated by monks, the Book of Columba, as it is also called, serves as the best representative sample of gospel books of its time .Although its content is mainly Christian, its has influenced many different aspects of history, such as art and especially calligraphy because of its elaborate illustrations and ornamentation.The…

“Wake up,” my mom said. Then she politely tapped my shoulder. It was 6:00 am, and it would be my first day of middle school in the United States. Months before my dad had taken us from Mexico to Paramount a small city in Los Angeles County “I don’t want to go” I said to my mom,” you have to go that’s why we came here for.” That morning I was scare of staring school in a new country, with people speaking a different language that I did not even know a word of. After waking up my mom, I…