Bioinformatics

P. aeruginosa is a gram-negative non-sporulating bacillus that belongs to the phylum of Proteobacteria. It can adapt and thrive in different niches, ranging from water/soil to plant/animal tissues. Even though it is commonly found in the environment, it frequently causes infections in plants and animals (1). Luke (1862) was the first to report P. aeruginosa in infections as he observed rod-shaped particles in blue-green pus of some infections. This coloration is a hallmark of P.…

In 1890 the novel, A Study in Scarlet, written by Arthur Conan Doyle introduced the character of Detective Sherlock Holmes. Doyle would go on to write 60 stories about Sherlock Holmes. The Sherlock Holmes stories have long been credited as an influence on forensic science thanks to his character’s use of investigative methods such as matching fingerprints, serology, ciphers, trace evidence, including footprints. 130 years later, this is old news, but a key component to investigations today.…

1-[Cyano(4-methoxyphenyl)methyl]cycloalkanol (VF-I) synthesized through the reaction of p-methoxyphenylacetonitrile and cyclohexanone using aq. NaOH and tetrabutylammonium hydrogen sulfate as PTC. NaBH4 and CoCl2 in 2:1 THF: H2O used for the reduction of VF-I to obtain 1-(2-amino-1-(4-methoxyphenyl)ethyl)cyclohexanol (VF-II). Further, venlafaxine derivative of the amino acid -carboxamide (VFCA-I toVII ) synthesized through coupling of N-Phthaloyl amino acid chloride derivative (2a-2g-…

Analysis of soil chemical and biological properties Soil chemical analysis and enzymes like urease, protease and nitrite reductase were carried out in the soil analysis laboratory, Guangxi University, Nanning, Guangxi Province, China. Soil pH analysis (soil:water = 1:1) performed by pH meter and Soil organic carbon was determined by dichromate oxidation (Walkley and Black, 1934). Total N in soil was determined by Semimicro-Kjeldahl method (Bremmer and Mulvaney, 1982). Nitrate nitrogen (NO3−-N)…

Whereas proteins functions cannot be understood without determining their three-dimensional structure. Protein structure prediction problem is one of the major challenges in bioinformatics and molecular biology[2]. The classical techniques for structure prediction of proteins are X-ray crystallography[43], [44] and Nuclear Magnetic Resonance (NMR) [45], [46]. But, these methods are expensive, time-consuming, and laborious, where…

Informatics is a technology term in today’s health care system. It deals with the science of computer information systems. When speaking of informatics in relation to an academic field, it involves the practice of information processing and engineering of information systems. The science of informatics according to American Medical Informatics Association (AMIA), 2015, “drives innovation that is defining future approaches to information and knowledge management in biomedical research, clinical,…

Gene traps are the plasmid or retrovirus-based vectors having a reporter gene that is only expressed when integrated in a functional gene. They were originally developed for the study of insertional mutagenesis in mouse. The gene traps were used to identify and characterize genes which were regulated by exogenous stimuli or during development process. The gene trap is a process which makes it possible to identify genes that gives rise to phenotypic effects when they are switched off, and also…

CHAPTER 1 INTRODUCTION Since time immemorial microorganisms have played a significant role in the life of mankind owing to the production of the myriad enzymes and secondary compounds that they synthesize (Demain 1981). These micro organism live in a highly diverse environment ranging from hot springs to deep ocean sediments. In the hyper diverse environment where one can find a specialized and unique biological niche is the intracellular space between cells of higher plants acts as suite and…

August 22, 2014 Dr. Caryn Ito Associate Scientist Sprott Centre for Stem Cell Research Ottawa Hospital Research Institute Ottawa, ON Dear Dr. Ito: Enclosed is my work-term report entitled Summer 2014 Work-Term Report: My experiences as CO-OP student working in the Ito/Stanford Lab at the Ottawa Hospital Research Institute. It is a description of the workplace, sepsis and the Ito/Stanford Lab’s sepsis project, my role in the project, and the skills that I have obtained during this placement…

1. Introduction Plants are crucial to the health and wellness of humanity. Over thousands of years, in an attempt to establish themselves within a single region, early humans began to use cultivation techniques, which is now called agriculture. It was with the advent of farming - when men realized that planting seeds resulted in germination - that societies began to develop more than 12,000 years ago. Communities then started to grow plants to feed them, and over the years, they developed a…