Bioinformatics

coli and a mutated plasmid. This lab group got mutation 1. The groups then used a program called Putty to view the bioinformatics program Muscle. Through Putty, each plasmid's DNA was sequenced to the base pair nucleotide letters. This enabled the groups to view the discrepancies between the genetic codes. Putty was opened on lab computers on the first day of this lab. Next…

Over the past few decades a lot of biomedical information has been gathered (in the form of images, signals and sequences) and there is a great deal of knowledge that can be gathered from the raw data \cite{manyika2011big}. The biomedical imaging data consists of magnetic resonance image (MRI) \cite{edelman1993magnetic, ogawa1990brain}, radiographic image \cite{hsieh2009computed, chapman1997diffraction}, positron emission tomography \cite{bailey2005positron} and others. Biomedical signaling data…

application and tools that have helped in the improvement of humanity. One of the many Biology branches, that is currently helping in medicine and other areas related to health, and biology, is the Bioinformatics. The way it is doing it is by improvement and be able to sequencing DNA (genome). Bioinformatics can be describes as a science tool in which use computer technology to analyze, collect, “storage, and retrieval of genomic data” (American Heritage Dictionary). There are different ways to…

metabolic responses to nutrition interventions or health conditions, associated with the animal performance and quality of its derived products. I use a combination of modern analytical chemistry (liquid/gas chromatography and mass spectrometry) and bioinformatics (univariate and multivariate statistical analysis) to measure metabolites in biological samples to provide insights on physiological or pathological status and nutrition effects. More specifically, I am interested in characterize the…

Chromatin Immunoprecipitation and Sequencing (ChIP-seq) of Heat Shock Factor (HSF) Temporal Binding Sites during the Critical Period of 8-12 Hr After Egg Lay (AEL) in Drosophila melanogaster Embryogenesis Background Heat Shock Response was discovered by the Italian scientist Ferrucio Ritossa, when he saw puffs in the Drosophila polytene chromosome on inducing them with heat. It was later discovered that this Heat Shock Response was regulated by a transcription factor called Heat Shock Factor…

break down the molecules. For example, it is important in humans, so it can break down all the starch by hydrolysis. This helps the human to make digestion. Many food has a big quantity of starch and a small amount of sugar. According to the bioinformatics study, the sequence…

believe current methods are reaching the technical acuity needed to address this question. This includes gains in high-throughput sequencing, digital qPCR, enhanced genetic tools for Drosophila melanogaster, better mapping of genomes and a surge in bioinformatics tools to analyze gene expression. While I have previously looked at the broad gene expression differences, this data set is lacking the more subtle analysis of different transcript abundance of specific genes. It is known that a…

campus. Secondly, all Gatton students take college courses with WKU students. This allows us to take challenging course loads in subjects that interest us. For example, I am interested in Biology and I have been able to take exciting classes like Bioinformatics and Neurobiology. Gatton…

The Process of Ligation, Transformation, and Isolation of EGFP cDNA Into pET-41a(+) By Using Miniprep, Restriction Digest, PCR, and Bioinformatics To Ultimately Create Recombinant Expression Plasmids. INTRODUCTION: Scientists in this day and age are now able to change DNA and create recombinant DNA, which is a huge indication of great possibilities for the future of genetics. The goal of these lab experiments was to be able to create recombinant DNA with the insert of egfp gene. EGFP also…

components of neural circuitry for establishing long-term memories. The proposed project addresses this important problem by employing a multidisciplinary approach in which we systematically combine tools of imaging, state of the art genomics and bioinformatics, molecular biology, functional analysis and electrophysiology. The proposed research is highly significant due to the following six major reasons: (1) Translation is affected in several neurodegenerative disorders. Several studies have…