Beta-lactamase

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    Catalase Test Paper

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    indeed Staphylococcus aureus within the throat. In the collection and isolation of the mixed culture from the nose and throat there were seven different test that aided in conformation of these two samples, which was the MSA fermentation plates, the beta hemolysis of the blood and DNA agar plate, the coagulase test, Antimicrobial substibility, the characteristics that were viewed under the microscope, and the catalase test. Out of these test, the determining factor in detecting positive or…

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    action of that drug. One antibiotic that is commonly used in outpatients is penicillin. The mechanism of action for penicillin is binds to the transpeptidase enzyme complex. The transpeptidase enzyme complex is used to crosslink peptidoglycan. When the beta- lactam is bound to the transpeptidase enzyme complex the crosslinks on the outer membrane can’t form. This makes the bacteria much more susceptible to environmental conditions and can be lethal to the bacteria. The lack of an outer membrane…

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    Ceftazidime-Avibactam

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    We see that the media today is increasingly obsessed with the end of the world. Whether it is by an array of simultaneous natural disasters, nuclear wars, EMP attacks, or more recently the Zombie Apocalypse. It seems that everywhere we go we cannot escape the end of the world hype. However, all of these things have one thing in common; they are visible. They are something that we can visibly see coming. Imagine if instead of having a visible apocalyptic nightmare we had one that was silent. One…

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    E. Colo Cell Lab Report

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    whether there was a successful transformation in the bacterium 's cells. If there is colonial growth of E. coli in an ampicillin environment then the plasmids triumphantly transformed the cell and as a result were able to create either the enzyme beta-lactamase, for the pUC18 transformed cells, or the enzyme luciferase, for the lux transformed cells, which would counteract the effect of ampicillin on the cell wall of the bacteria. There were many errors in this experiment. Only two of the agar…

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    Ampicillin Experiment

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    various organisms due to a high integration potential. E. coli research is often used to study gene expression and has a high potential of being applicable to humans.2 For these reasons, E. coli is often used in research experiments. Ampicillin is a beta-lactam antibiotic…

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    binding to their active site. Thus inhibiting cell wall synthesis which stops cell division and causes lysis due to fragility of cell wall and high osmotic pressures. Aeromonas spp. contain the genetic information that codes for the synthesis of Beta-Lactamase an enzyme which inactivates the β-lactam ring via hydrolysis causing a resistance to ampicillin (Li, Chan, & Chen, 2016). Therefore, Aeromonas spp. with this resistance no this organism can grow in the presence of…

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    Case Study Cellulitis

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    ampicillin-sublactam intravenously (1). Ampicillin is a derivative of penicillin. It works by binding to bacterial cell wall and leading to bacterial death. Sublactam works in conjunction with ampicillin by increasing the drug’s resistance to beta-lactamase. Use is indicated in cases of skin and skin structure infections (8). In the four remaining weeks, the patient received levofloxacin orally. Levofloxacin works by inhibiting DNA gyrase in bacteria, therefore preventing the bacteria from…

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    Genetic transformation is an important method, in molecular biology and genetic engineering, for transferring DNA amongst a variety of organisms. In Lab five, my lab partners and I used calcium chloride to make the bacterium cell walls more permeable and a heat shocking method to introduce the pGLO plasmid in the E.coli bacterium so that they may exhibit ampicillin resistance. The Goal of the experiment was to observe whether or not, given one of the four specific conditions, the pGLO plasmid…

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    Short Bowel Syndrome

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    Definition and Causes of Short Bowel Syndrome The patient in this case study was immunocompromised due to Short Bowel Syndrome (SBS) which predisposed her to discitis infection. SBS is a disorder that hinders the absorption of nutrients and fluids usually after extensive small intestine resection (Tee, Wallis, & Gabe, 2011). An average person’s small intestine is 600cm long (Sundaram, Koutkia, & Apovian, 2002). In SBS, the small bowel has been shortened at least 70 percent (Sundaram et al.,…

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    aureus in nosocomial settings” (5) “MRSA strains have been reported to have a negative surface charge.”(10) MRSA produces large amounts of beta-lactamase. “The bacteria has teichoic acid at the cell surface.”(10) Methicillin resistance is enhanced by NaCl. People are more at risk for an MRSA infection if they have a low immune system or have an entry points for bacteria to enter the body. Sores…

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