Beta-lactamase

the Beta Club website, "Beta Leadership Camps offer experiences that build confidence and character. Students will be motivated, inspired and changed as they interact with other Beta members from across the nation and form bonds that last a lifetime." Conventions are also excellent as well. Not only do they provide a healthy competitive environment to students so they can work to improve themselves, it also inspires students who aren't even involved in the organization! According to one Beta…

Miami, Florida Major: Exercise Science Instagram: hitmanlive5 Students across UWF are likely to see Jose’s positive and bright attitude around campus. What they might not know is how heavily involved he is. Jose is a member of the Beta Beta Phi chapter of Phi Beta Sigma, the media chair for Collegiate 100, the Vice President of the Haitian Student Organization, and is going be a facilitator for the Leadership Gauntlet this upcoming spring semester. However, Jose’s biggest focus right now is…

Joshua Wilks is an Information System Technology major at Morgan State University. He has worked for Johns Hopkins Bloomberg School of Public Health as a research assistant for the DIVE program. The program researched liquor stores in baltimore city and how more than half need to be closed down because there are too many. In this program Joshua Wilks was a team leader and aided in the project being completed a month earlier than expected. He is prepared to work with customers after working with…

Lael Brainard was born in 1962, in Hamburg Germany to parents Al and Joanne Brainard. Al Brainard was a U.S. foreign-service officer who was stationed in Cold War Germany and Poland. Dr. Al Brainard received his bachelor’s degree from the University of Connecticut and his doctorate in Political Science from the University of Washington. He was one of the first US officials to develop relationships with Polish dissident movements that were instrumental in Poland’s transition to democracy. Ms.…

The drugs stop the bacteria from correctly building their cell walls. There are two parts to the wall-assembly process. They are synthesizing new strands of linked sugars. Then linking them into the matrix that is growing. Beta-lactam drugs work by preventing the enzymes that build cross-links form doing their job, weakening the wall. The wall won’t stay intact, so the bacterial cell explodes and dies. The framework of the bacteria vs.penicillin battle is recognized, but…

resistance against beta-lactams, namely,developing PBPs with lower affinity for beta-lactam, reducing access to PBPs, or developing enzymes which act against the antibiotics, known as beta-lactamases (Rice 2012). The first method,that of producing new PBPs is most common in gram-positive bacteria, such as Staphylococcus aureus, which produces PBP2a,or Enterococcus faecium, which produces PBP5 (Rice 2012). Gram-negative bacteria however favour the production of beta-lactamases (Rice 2012). These…

In transformation a prokaryotic cell is altered by the uptake of foreign DNA from its surroundings (Reece 579). This process occurs in nature but can be artificially induced in a laboratory by a method called heat shock. Prokaryotic cells that have been artificially transformed by the foreign DNA can then be calculated for efficiency of transformation. pGLO plasmid was an excellent choice of foreign DNA, due to its ability to alter the phenotype of the prokaryotic cell. Green fluorescent…

Penicillin is a widely used antibiotic, favored for its effectiveness for multiple bacterial infections while keeping human cells healthy and abundance. It wasn’t always this way, penicillin needed an entire lab team to discover how to purify and produce enough to cure infections. First, it needed to be discovered. The man who did that was Dr. Alexander Fleming, a bacteriologist at St. Mary’s hospital. He had returned to his lab to find that his improperly cleaned lab station had the mold…

Extended spectrum β-lactamase (ESBL) enzymes have been stated in species of gram-negative bacteria. The ESBL enzymes are commonly plasmid mediated and are able of hydrolyzing and inactivating a wide-ranging variety of β-lactams, including third-generation cephalo-sporins, penicillin and aztreonam, but are vulnerable to β-lactamase inhibitors for example clavulanate, sulbactam and tazobactam However, those inhibitors inhibit…

contained beta lactamase which made the cell resistant to ampicillin. It did not end up glowing under UV light because the gene for the green fluorescent protein was repressed by the araC gene found on the DNA plasmid. The hypothesis about the +pGLO plate containing LB, ampicillin, and arabinose was correct because the bacteria did end up growing and glowing under UV light. The bacteria grew because there was a source of food present to help the bacteria grow and reproduce and there was beta…