transformation. Plasmids such as lux and control pUC18 allow the cell to transform in order to grow and survive, while both contain ampicillin-resistant genes. Both the lux and control pUC18 plasmid were added to the competent bacteria in the cold ice bath. After the ice bath, the cells were immediately transferred into a heat shock. Heat shock expands the cell allowing the plasmids to enter the host cell. The solutions were later poured into six different agar plates. Three of the agar plates…
Self centered bold lustful who other than the wife of bath the wife in my opinion was not unlike many women we see in today's culture she did not take commands from anyone especially a man and thought men were there wife's slaves, this in itself makes her such an eye-opening character as a woman myself I find her personality rather intolerable and disgusting. The wife also known as Allison was portrayed as a religious woman in the general prologue she was known to go on many pilgrimages…
Next, we will add 2 mL of sodium hydroxide to each test tube. This will raise the pH of the solution to alkaline levels. Next, we will place one of each test tube in the three different temperature water baths for 30 minutes. After 30 minutes, we will remove the test tubes from the water baths and add six drops of Biuret reagent to each tube and mix thoroughly. The Biuret reagent will test for the amount of peptide bonds present in the egg whites. Then we will take a picture of the results…
Great Pets to Own There are so many types of animals that would be a wonderful pet, but I found that a cat, dog, hamster, and fish are the top ones that people choose to adopt. So why are these cute little pets the ones to be chosen? Here’s why Cats are a great pet because most are easy to take care of. When it comes to cats, you don’t need to take them outside to use the bathroom, all you need is a litter-box and a week or two and they be good to go. Most cats learn fairly easy to use the…
experiments. 25. All of the equipment used were washed and cleaned carefully, and returned to the appointed places. • Each of the 5 different independent variables was repeated for 3 times as 3 trials. • The conical flasks were placed in the water baths for two minutes before the experiment was started. • Hot water and ice were added to the plastic container whenever there was change in…
such as giving a cranky resident a be bath. Anyone that has done this knows that it is not a easy task to complete. C.N.A’s do not get paid enough or recognized for their hard work, unlike doctors and nurses. The items you will need are: three washcloths, a bed basin, a bar of soap, lotion, bedside table, and gloves. Before you do anything you will need to put on your gloves. Next you will hace to persuade the resident to let you perform the bed bath. You can do this by offering a soda…
Chaucer is an interesting and fascinating book. It explores the tales of the people going on a religious journey, “The pardoners tale” and “wife of bath tale”are both compelling and engaging books.The movie “The Wolf of wall street” is similar to the plot and storyline of “The pardoners tale” and “The wife of bath”. The pardoners tale. The wife of bath and The wolf of wall street explains the allegory in the life of the characters. The pardoner loved to drink a lot, he even had a drink before…
4 Tips to survive Daylight Savings with Children “Daylight Savings”… are curse words for moms along with “Time Change” and “Fall Back”. Two times a year, we feel the need to mess with our sleep schedules that we spend the rest of the year working to perfect so that mommies can have alone time, or clean up time, or even wine time., no judgment! I’m originally from the Saskatchewan myself, one of the few places in the world next to Hawaii that does not observe daylight savings. Our reason was…
Tale and organized in support of the orthodox position of the Man of Law as tempered by the sensuality and worldliness of the Wife of Bath. The Franklin's position on marriage differs from the Man of Law's only in that it takes a far less austere view of this world's joys. The Franklin strives for something in between the complete sovereignty advocated by the Wife of Bath and the patience suggested by the Clerk. The marriage in The Franklin's Tale is one of mutual consent, mutual obligation, and…
Leonardo da Vinci’s “Virgin of the Rocks” and Mary Cassatt’s “After the Bath” are two pieces of art I will be comparing and contrasting. [HELP] “Virgin of the Rocks”, also known as, “Madonna of the Rocks” are two pieces of art composed by Leonardo da Vinci, who was a Renaissance artist. They were created in return for a commission by the Fraternity of Immaculate Conception on April 25th, 1483. This painting was going to be in the Church of San Francesco Grande for an altarpiece that would hang…