Bacillus

Laboratory Analysis Justyna Sudyka 1. a. Clostridium sporogenes grew throughout all of the thioglycollate broth, but there was more growth towards the bottom. The aerobic TSA + glucose plate did not have any growth, but the anaerobic plate did have growth. Escherichia coli grew throughout all of the thioglycollate broth as well, but the organism was clustered slightly more towards the top. There was growth in both aerobic and anaerobic TSA + glucose plates. Micrococcus luteus only grew at the…

First the unknown tube was isolated. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. This was done using the streak plate method, which is described in the lab manual. The bacterium were incubated and grown at 48 hours. Upon observation the isolation plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the…

Bacteria also known as prokaryotes are very ancient single-celled organisms; fossils show that they were widespread 1.5 billion years ago. Today bacteria make up the vast majority of prokaryotes on earth due to the unfavorable ability to adapt quickly to the surrounding environment. Bacteria have the capabilities to survive in areas of high heat, extreme cold, or even very acidic or alkaline conditions. This ability is used frequently to fend off the effects of antibiotics and antibacterial;…

Gas gangrene belongs to the genus Clostridium (Carlisle 2014). It’s a non-motile, gram-positive, anaerobic, and spore-forming bacillus (Carlisle 2014). The most common etiologic agent that causes gas gangrene is called Clostridium perfringes. This can enter your body through open wounds and eating poorly cooked meat. Since C. perfringes is an anaerobic bacterium, it uses energy by using anaerobic respiration and Nitrate as an electron acceptor. When the bacterium is around Nitrate, it will…

Mannitol salt agar. All three plates were studied, a gram stain was performed according to the referred laboratory manual noting their color and morphology, which was recorded in the journal. Gram-positive purple coccus bacteria and gram-negative rod/bacillus were identified using the microscope. The purpose of streaking on the MacConkey is to see if gram negative is lactose fermented or not and on the Mannitol salt agar if gram positive microorganism is able to ferment sugar. There were two…

Chalcone scaffolds had attracted many researchers to investigate their synthetic routes, potential biological activities and their roles as intermediates for naturally occurring flavanoid skeletons. This study focused on the synthesis of chalcones bearing O-, C-prenyl and chromane as substituents. The target compounds were purified by chromatographic methods and structures of all compounds were determined by using IR, NMR and MS spectroscopies. The prenylated precursors were synthesized…

The most common bacterial infections that are gram positive are staphylococci which produce enzymes such as catalase which break down hydrogen peroxide bonds, bacillus cereus - food poisoning, bacillus anthracis - anthrax and clostridium tetani - tetanus. Penicillin is an antibiotic that prevents gram positive bacteria from forming peptidoglycan. Peptidoglycan plays a key role in the cell wall. If peptidoglycan was not present, then the bacterium would swell and burst due to the high internal…

Since the species of the American pygmy shrew is so widespread, there are a variety of different threats to each population (ANHP, 2017). The species has a global ranking of G5 and a National ranking of N5 which list the species as secure with no apparent threats to survival (Gervis, 2015). Although, specific states have release reports of overlapping threats to smaller populations. S1 states such as Alabama , Idaho, Massachusetts, and Wyoming are closely monitoring populations. Many of these…

Cells are the basic units of life. Each cell has different functions and properties that make life diverse. In this experiment, we had to deal with infections in two people. In order to treat the infections, we had to conduct a Grams’ test to figure out what kind of infection the children had and then identify the antibiotic that would best treat the infection or if they were antibiotic-resistant bacteria. For this experiment, my hypothesis is that both culture’s A and B will not be…

pollution, greenhouse gas release, and soil break down. Stronger producing because of GMOs could allow farmers to dedicate less land to plants. Also, farmers are already growing corn, cotton, and potatoes without spraying the bacterial insecticide Bacillus thuringiensis because the plants produce their own insecticides, according to the Food and Agriculture Organization of the United Nations. Seeds are genetically changed for many reasons, which include improving resistance to insects and making…