Bacillus subtilis

Laboratory Analysis Justyna Sudyka 1. a. Clostridium sporogenes grew throughout all of the thioglycollate broth, but there was more growth towards the bottom. The aerobic TSA + glucose plate did not have any growth, but the anaerobic plate did have growth. Escherichia coli grew throughout all of the thioglycollate broth as well, but the organism was clustered slightly more towards the top. There was growth in both aerobic and anaerobic TSA + glucose plates. Micrococcus luteus only grew at the…

Introduction: The effectiveness of Antimicrobial agents against Microorganisms Population of microorganisms or growth are killed and/or inhibited by natural or synthetic chemical antimicrobial controls (Madigan, et al 2012). Antimicrobial agents are grouped according to their efficacy against microorganisms. Bactericidal that kill and bacteriostatic prevent and/or reduce growth (Madigan et al, 2012). Harmful pathogens are targeted with different antimicrobial agents according to their toxicity…

The purpose of this report is determine whether the natural product; vinegar, is better than man made chemical products; Domestos and Pine O clean. This will be determined by the results of the experiment which product has created the largest zone of inhibition. To determine which of the three disinfectants are the most effective depend the one with the largest inhibition zone. Bacteria are tiny microorganisms that colonise together and are found in every living organism. Bacteria is a type of…

ammonia, nitrogen gas and nitrous oxide (N2O) is called denitrification. This process ends in the release of gaseous nitrogen into the atmosphere and thus completes the nitrogen cycle. A number of bacteria such as Pseudomonas denitrificans, Bacillus subtilis and Thiobacillus dentrificans are involved in this…

coli), Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis)), Nutrient agar plate Experiment 1.5 commenced by dividing the Nutrient agar plate into four equal segments. Aseptic technique was used to pick up a sample of broth culture using the inoculating loop. The sample was then inoculated onto…

Chalcone scaffolds had attracted many researchers to investigate their synthetic routes, potential biological activities and their roles as intermediates for naturally occurring flavanoid skeletons. This study focused on the synthesis of chalcones bearing O-, C-prenyl and chromane as substituents. The target compounds were purified by chromatographic methods and structures of all compounds were determined by using IR, NMR and MS spectroscopies. The prenylated precursors were synthesized…

The aim of the data analysis exercise was to determine genetic relationships between the HSP70 amino acid chains of fourteen different organisms. The data produced was an identification table, a sequence alignment diagram and a cladogram. In cladograms the branches are not representative of the evolutionary time that has occurred. Heat shock proteins (HSPs) are in a class of proteins called molecular chaperones. (Kiang, J.; G, Tsokos. 1998). They are present in both eukaryotes and prokaryotes,…

B Sridhar et al (2012) studied Purification and Characterization of Biosurfactants. The emulsifications of biosurfactants were checked in various oils such as olive, coconut, sunflower and rice bran oils, the emulsification activity of biosurfactants. Pseudomonas aeruginosa had higher emulsification activity than Saccharomyces cerevisiae. While checking the sugar and lipid content in biosurfactants by phenol sulphuric acid method and vanillin reagent method, found high in Pseudomonas aeruginosa…

of Ag+ with thiol group played an essential role in bacterial inactivation. Silver Silver nanoparticles have inhibitory activity against Gram positive bacteria and Gram negative bacteria as well. Several experiments conducted against E.coli, B. subtilis, SS. aureus, methicillin-resistant coagulase-negative staphylococci, vancomycin-resistant Enterococcus faecium, ESBL-positive K pneumonia, S. typhi, V cholera to analyse the inhibitory effects of silver nanoparticles.The antibacterial activity…

The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if…