Bacillus cereus

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    1. Summary of how I identified Bacillus cereus. a. First, I performed the gram stain test, I then went on to do the spore stain. b. The results I obtained from the gram stain were gram positive rods, I could tell they were positive by the purple color to them and the rod shape by the comparison provided in the lab manual (Wilkosz, Peterson). After I confirmed the results I performed the spore stain, which indicated that my sample was positive so it contained endospores. The sample should have shown that there was a red vegetative cell and a green stained endospore (Wilkosz, Peterson), but I must have over rinsed with water because the cells appeared clear with an outline, and the spores that were seen appeared as black dots. c. The first test performed was the gram stain, the purple color indicated that it was gram positive, this removes all gram negative possibilities including Pseudomonas aeruginosa, Klebsiella pneumonaie, and Proteus vulgaris. Since I also observed that the bacteria were rod shaped, it excluded the cocci specimens Staphylococcus aureus and Streptococcus pyogenes. After performing the spore stain and noticing that there were spores, which concluded that it was Bacillus cereus. d. I had Unknown plate #12 which I determined…

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    disposable gloves was significantly better compared to the use of bare hands and hands containing reusable gloves” (Brizio et.al.,2014). Consequently, the use of gloved hands when handling certain foods, specifically raw meat, does offer protection against toxic bacteria though even with this type of armor the threat of poisoning still transpires making gloves only a slight reassurance. There needs to be a definite more secure means of protecting oneself. The first influential step we as a…

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    their choice. Unknown test tube #25 was obtained and streaked onto two agar plates to obtain individually isolated colonies. The gram-staining procedure was used to determine organismal morphology, resulting in a gram-positive cocci and gram-negative bacilli. A sucrose utilization test was performed and narrowed the possibilities of the gram-negative bacilli to Proteus vulgaris and Enterobacter cloacae. Then a Methyl Red and Voges-Proskauer (MR-VP) test was done to confirm the identity of the…

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    Gram Unknown Lab Report

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    In order to determine the identity of two unknown bacteria, I performed a series of experiments over several days. To begin this process I created a dichotomous key that I utilized as a guide to perform a series of inoculations, while using both selective, and differential media. The series of inoculations eliminated bacterium based on test results until the correct bacteria were both positively identified. On day one of this process I received two bacteria, one labeled 7A and the other…

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    2. Materials and Methods: The keratin was extracted from Chicken feather which were collected from the local market of Raipur (21.14°N 81.38°E) Chhattisgarh, India. The 91% food grade alginate (sodium salt form) was purchased from Loba Chemie. The β-mercatpoethanol (mol. wt. 78.13) from LOBA Chemie, sodium dodecyl sulfate (SDS) (mol. wt. 288.38) and urea (mol. wt. 60.06) was purchased from Merck, and ethanol was purchased from Sigma-Aldrich. Antimicrobial strain of gram negative bacteria…

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    Anthrax Research Paper

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    Anthrax is caused by spore forming bacterium. It mostly affects animals. Humans can be infected if come in contact with infected animals. It was most common with sheep and cattle. In 1881, Louis Pasteur focused his attention on anthrax. Louis Pasteur performed public experiments in which he injected one group of animals with an anthrax vaccine he developed (9). Pasteur gave 25 shots of anthrax vaccine he had created with a weakened anthrax bacteria (8). Pasteur gave both rounds of the vaccine…

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    DISCUSSION Unknown #297 was collected from a urine sample from a patient with a kidney infection, named Doris. There have been several tests that were performed at the University of Illinois clinical Laboratory to identify the unknown. Unknown #297 was identified to be Bacillus subtilis with the various tests that were conducted. Most of the results that were observed in the laboratory matched the expected results for the bacterium. All of the morphological characteristics results matched…

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    Bacillus anthraces is an intense irresistible malady brought on by Bacillus anthraces, a spore shaping, gram-positive bacillus. Related malady happens most much of the time in sheep, goats, and dairy cattle, which get spores through ingestion of polluted soil. People can get to be tainted through skin contact, ingestion, or inward breath of B. anthraces spores from contaminated creatures or creature items (as in "fleece sorter's infection" from introduction to goat hair). Individual…

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    Anthrax Research Paper

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    Anthrax is an acute infectious disease caused by the spore-forming bacterium Bacillus anthracis. This zoonotic disease occurs naturally in many areas of the world, most commonly in agricultural regions with inadequate control programs for anthrax in livestock. The anthrax spores are the major form of the bacterium in the environment and anthrax is contracted via spore uptake. Resistance to dehydration, heat, ultraviolet light, gamma radiation and many disinfectants makes anthrax spores ideal…

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    Corn, beyond doubt, is one of the biggest stories in human history. The “ruler” of the entire American food chain is corn. From farmyards to supermarkets, countless types of food ingredients and even forage are corn-derived. In 2007, the States alone produced 13 billion bushels of corn. Such massive reap is attributed to corn-farm subsidies by the government, a “legacy” of the Great Depression which gives corn an unnatural edge over other kinds of crops. The policy is, in fact, skewed in favor…

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