Assay

wasn’t performed. Normal immunologic tests (normal values of immunoglobulin A, M, G, Ag, normal phagocytic activity of neutrophils and nitro-blue tetrazolium test, a sufficient level of circulating immune complexes and negative immunofluorescence assay of Human Immunodeficiency Virus (HIV) ruled out a possible diagnosis of primary or secondary immunodeficiency, ruling out the possibility of haematological disease. Changes in the chest computer tomography (CT) scan revealed a round mass with…

Explanation of a microbiological algorithm for Hospital-acquired and Ventilator associated pneumonia. This algorithm is intended to deal with the microbiological aspects of hospital-acquired and ventilator-associated pneumonia. The severity of the illness is important in determining when to collect the diagnostic samples and which antimicrobials to administer due to which organisms are present. There are many prognostic scoring systems used to suggest the severity of the disease in patients…

Introduction Each year, millions of Americans are infected with Clostridium difficile infections (CDIs), accounting for approximately 15,000 fatalities per year. When the diarrhea caused by the infection becomes severe, life-threatening complications may arise. Treatments are usually done with antibiotics, but with the rise of a newer and more virulent strain of C. diff, medications have become less effective. So to help combat the CDI epidemic, an old treatment called fecal microbiota…

Francisella tularensis is a bacterium that has been recognized to be an agent responsible for the disease known as tularemia. This disease, in essence, sends the immune system of the organism into a hyperactive state, sometimes referred to as hypercytokinemia due to the elevated levels of various cytokines, which if not treated properly could lead to extremely high fevers, inflammation of various tissues, and (if contracted through aerosol form) the death of the infected organism. Specifically,…

Introduction The Gram stain is a valuable tool to microbiologists in identifying the cell well composition of prokaryotic species. The underlying process revolves around a series of chemical dyes, and their propensity to bind to peptidoglycan polymers in the cell wall. The results allow scientists to infer properties about the outer coating of an organism and is often the first step in identifying an unknown species. For the purpose of our experiment, the results of the Gram stain will be as…

Alkylated polycyclic aromatic compounds (alkyl-PACs) are the major aromatic constituent of total PAH in crude oil and originated from incomplete combustion process. Whereas benzo[a]pyrene (BaP) is a well-known human carcinogen, dibenzothiophene (DBT) and retene (Ret) are not; however, all three compounds are important environmental pollutants. Their toxicity is not yet characterized. In this study, we used BaP (parent PAH), DBT and Ret (alkyl-PACs) to elucidate the mechanism of neurotoxicity…

separates and other specimens from accidental or deliberate release. The inactivation of specimens, reliant on the detection protocol used, should be completed under BSL3 conditions. For non-inactivated samples, RT PCR and enzyme-linked immunosorbent assay testing can be performed at a BSL3 laboratory. If samples have been inactivated (i.e. cell lysis) RT PCR and ELISA testing can be performed at a BSL2 laboratory. Laboratory diagnosis: Laboratory results should…

The logos, ethos and pathos of these genres is self-evident, granted the reader has the required conceptual knowledge necessary for understanding the lexis and text of said genres (Wadsworth guide to research p.183). Research articles:The research article fulfills its logos appeal because of its well-structured and logical organization allows meaningful communication. Research articles, typically, tend to prove or disprove a hypothesis and finalize their results in the form of a conclusion;…

Collaborators sent a number of native North American cactus moth larvae from different locations in Texas, Arizona, and New Mexico to the containment facility in Gainesville for non-target assays. So far no parasitization occurred in no-choice, non-target host testing of the other Lepidopteran species. Individuals from colonies of Melitara prodenialis from ARS lab, Tallahassee were used in non-target testing. Out of 501 M. prodenialis larvae…

patients test positive for this mutation. The genetic mutation is present in ECD lesions only, not in the “germ line,” which is to say that it is not a hereditary problem. This testing should be done using immunohistochemistry and an ultrasensitive assay as there have been a large number of false negative findings associated with BRAF testing of ECD patients. However, definite diagnosis of ECD is established only once CD68(+), CD1a(−) histiocytes are identified within a biopsy specimen. To…