Assay

Genetically modified organisms or commonly known as “GMOs”, are the product of a process where certain genetic information is extracted from one organism’s DNA and artificially placed into the genes of another organism. Since the genes are from two different species, the organism would be referred to as being transgenic. That is all describing a process called genetic modification or “GM” for short. This unnatural process leads to the production of plants, animals, bacteria, and viruses that…

The World Health Organization, also known as W.H.O, is one of the dominant organizations in the world that dedicate themselves to improve individuals and community health across the globe. It is the specialized agency of the United Nation, which direct and coordinate authority for health matters within the system (1). W.H.O is responsible for providing health services and technical support to countries across the globe, promote and coordinate with the international research works on various…

• The % RSD values for Ceftriaxone and Tazobactam were found to be < 2 %, which indicates that the proposed method is repeatable. The low % RSD values of repeatability of assay (0.258-0.210 %), inter day (0.080-0.345 % and 0.045-0.321 %) and intraday (0.055-0.136 % and 0.055-0.090 %) variations for Ceftriaxone and Tazobactam, respectively (Table 2.03), reveal that the proposed method is precise. • LOD values for Ceftriaxone…

ա-conotoxins have become standard pharmacological reagents for investigating voltage-sensitive Ca2+ channels and are used to block presynaptic termini and neurotransmitter release. Conotoxins are also used in medical diagnosis; an immunoprecipitation assay with radiolabeled ա-conotoxin can be used to diagnose the Lambert-Eaton myasthenic syndrome (16), a disease…

1. ABSTRACT: A total of 552 serum samples of sheep, goats and cattle from different localities at Assiut governorate, Egypt were screened for qualitative analysis of the BTV antibodies using a commercial competitive ELISA (cELISA) kit. The results showed an overall percentage of BTV positive sheep, goats and cattle serum samples were 41.86%, 24% and 85.42% respectively. A highest percentage of seropositivity was found in Arab El- Awamer farm(100%), followed by Abnoub( 63. 64%), Sedfa(11.…

It is generally accepted that a virophage is a viral agent that infects other larger viruses. It is a dsDNA virus which utilizes the replication factories of other viruses to reproduce itself. Virophages are essentially parasitic in nature, much in the same way as traditional satellite viruses. It is not without controversy that distinctions between satellite viruses and virophages are described. The difference between the two often seem more semantic than factual and more supported by…

Chromatin Immunoprecipitation and Sequencing (ChIP-seq) of Heat Shock Factor (HSF) Temporal Binding Sites during the Critical Period of 8-12 Hr After Egg Lay (AEL) in Drosophila melanogaster Embryogenesis Background Heat Shock Response was discovered by the Italian scientist Ferrucio Ritossa, when he saw puffs in the Drosophila polytene chromosome on inducing them with heat. It was later discovered that this Heat Shock Response was regulated by a transcription factor called Heat Shock Factor…

Case Study 1 1. This article featured in Daily mail newspaper. It has been updated on 3rd of June 2010. 2. Tekmira is the pharmaceutical company involved in the research. 3. Ebola was the disease which was being treated. The key symptom of Ebola is haemorrhagic fever and the typical outcome of Ebola is 90% death. It’s transmitted via personal contact e.g. bodily fluid such as blood and saliva. 4. The company used a small interfering RNAs which hold the virus at bay until the immune system is…

Many corporations use animals not only in research but to test cosmetics. Everything from the traditional lab rat to non-human primates injected with diseases such as HIV can be found in scientific laboratories every day. Some corporations such as L’Oréal, M.A.C. Cosmetics, and many others use animal testing. Animal testing is used widely around the world. Using animals as test subjects is a form of animal cruelty and should be banned from all countries. One article states, “Animal testing, or…

3.3 Results and Discussion 3.3.1 Agarose Particles By microscopy observation, both ABT and CSS were activated by the Mix&GoTM solution with significant colour change on the particle surface. Seen from Figure 3-1 and 3-2, a dark green layer was formed outside the particle after treating with Mix&GoTM. By visual observation, the agarose particles turned from colourless to green, which supported the microscopy observation. The activated particles were also sent for zeta potential measurement and…