Assay

replication (Ahmadi 2011). Chlorothalonil is a commonly used fungicide in Canada for vegetable and fruit fields as well as lawns and turfs. Using a single-cell gel electrophoresis (SCGE) assay DNA damage was observed for chlorothalonil within a 0.2 to 1μm concentration range (Vigreux et al. 1998). Using an alkaline SCGE assay it was detected that chlorothalonil induced DNA damage in human lymphocytes, although it is not genotoxic (Vigreux et al. 1998). When introduced into the body in sizable…

in choroidal flat-mount. The content of vascular endothelial growth factor (VEGF) in the supernatants of ARPE-19 cell cultures was examined with the enzyme-linked immunosorbent assay (ELISA). The toxicity of menadione (VK3) and the protective effect of Borsub against VK3 were studied in ARPE-19 cells with cell survival assay. Autophagy activation in ARPE-19 cells by Borsub was examined with western blotting and immunofluorescent confocal laser microscopy.…

commercially available kit, MyBioSource, Inc., California, USA. The kit utilizing a monoclonal anti- PPAR-γ antibody and an PPAR-γ-HRP conjugate enzyme-linked immunosorbent process assay (ELISA) to determine the concentration of PPAR-γ in serum, plasma, urine, cell culture supernatant and tissue homogenates. Principle of the assay: The sample and buffer have been pre-coated with PPAR-γ-HRP conjugate. then incubation is carried out, followed by washing to remove the uncombined enzyme. Upon adding…

To correct the mutation in the gene that lead to the development of cystic fibrosis, a team of Yale researchers led by Dr. Peter Glazer (Chairman, therapeutic radiology), Mark Saltzman (Chairman, biomedical engineering), and Dr. Marie Egan (professor of paediatrics and of cellular and molecular physiology), the collaborative team operated certainartificial molecules identical to DNA known as Peptide Nucleic Acids (PNAs), as well as donor DNA, to recover the genetic abnormality.Professor Egan…

recent research, that troponins levels might be elevated in certain non-cardiac conditions such as pulmonary embolism, sepsis and renal insufficiency inter alias[42]. It has been noted in patients with ESRD that, without any symptoms or signs of ACS, troponin level is elevated. Such findings put clinicians in a dilemma in the realm of diagnostics and treatment. Renal and cardiovascular diseases are intricately linked showing a moderately high level of co-occurrence. It has been noted that…

Psychological Stress Test The acute psychological stress test we used was a ten-minute version of the Paced Auditory Serial Addition Test (PASAT) (Gronwall, 1977). This has been shown to reliably perturb both the cardiovascular system and salivary cortisol levels (Phillips et al., 2005, Phillips et al., 2006, Phillips et al., 2009) and demonstrate good test-retest reliability (Willemsen et al., 1998). In this task, the participants were presented, via a compact disc player, a series of single…

The first time I did a plaque assay my phage was at a concentration of 7.2*10^6 pfu/ml. The second time I did a plaque assay my concentration was fairly similar, a little lower, it was 2.8*10^6 pfu/ml. From this second round of purification, I picked a relatively isolated plaque and used that to do a serial dilution, from this…

following cigarette smoking or use of ammonia-containing detergents are some of the factors that cause pre-analytical errors. For these reasons, the existing literature [1,7] and ammonia assay manufacturers recommend: a) use ethylenediaminetetraacetic acid (EDTA) as a preservative, since heparin interferes in the ammonia assay; b) avoid serum, because during the coagulation process ammonia could be produced; c) collect the blood from the veins without stasis; d) prohibit smoking to the patient…

in purification, it was achieved by denaturing the non-recombinant E.Coli proteins. The Second step was much more beneficial to the total purification but the combination of the two steps achieved an even better purification of 4.6 fold. From this assay we can see that the correct enzyme has been successfully purified. Considering the SDS-PAGE results we can confidently say that the purification steps were effective at removing a large proportion of E.Coli proteins. However, not all E.Coli…

Viruses are particles that depend on a host cell they infect to reproduce and carry out metabolic functions. They are considered both living and nonliving. Viruses reproduce and mutate, therefore they can be considered living. However, they also lack organelles and cytoplasm, which is characteristic of a nonliving particle. Most viruses contain either DNA or RNA enclosed in a protein coat called a capsid, and upon infecting a host it induces the host cell’s replication machinery to reproduce. A…