Arginine

isotope labeling by amino acids in cell culture (SILAC) experiment whereby the Iscove’s modified minimal medium (IMEM) was custom prepared based on the ATCC formula without L-lysine or L-arginine. However, the complete light and heavy IMEM media was prepared in the addition of light and heavy lysine and arginine along in dialyzed FBS. The collected HL-60 cells were cultured in heavy IMEM medium for five cell doublings to achieve a complete isotope incorporation. The cultured medium was…

ACUTE KIDNEY INJURY – Acute Kidney Injury (AKI) is currently recognized as the preferred nomenclature for the clinical disorder formerly called Acute Renal Failure(ARF).This transition in terminology was meant to emphasize that the spectrum of the disease is much broader than a subset of patients who experience failure and require dialysis support .This nomenclature explains that renal failure occur as a continuum 1. AKIN recently defined AKI as “ functional or structural abnormalities or…

Mechanisms of the disease The Nephrogenic Diabetes Insipidus is firstly a rare disease to get or it can be inherited and the rate of getting it is 1 in 20 million which shows how rare this condition is. The cause is that the body has low levels of Arginine Vasopressin (AVP) and Antidiuretic hormone (ADH) which then cause the kidneys unable to concentrate the urine which is called polyuria which leads to excessive amount of urine being produced which leads to polydipsia. So when fluid levels…

Introduction With the pet owner being in complete control of their canine’s nutrition, the dog’s food can have a vast impact on any recovery. When friends and family are being treated for cancer they may have experienced many symptoms. Ranging from depression, loss of appetite, confusion, and exhaustion. Some effects from treatment can cause behaviors that are counterproductive in the recovery. For example, not drinking enough water because of sleeping throughout a day, can easily cause…

For hundreds of years dwarfism has been a fascination of society. Historically people with dwarfism were used in circuses and sideshows because people were in awe of their stature and proportions. Dwarfism is most commonly caused by the genetic condition Achondroplasia, which accounts for 70% of all dwarfism cases and occurs in one out of every 25,000 live births (Pauli, 1998). Achondroplasia (ACH) is one of many genetic anomalies which causes the condition dwarfism it is an autosomal dominant…

previous experiment. In addition, they replaced other codons such as; AGC, AGU, UUG, and UUA because these codons were not recognized in aminoacyl-tRNA synthetases. They used two codons AGG and AGA, the two rarest codons used because the amino acid arginine (AGG/AGA) compromise only two to four percent of the entire E. coli strand. This helped minimize the amount of changes that was done to the final E. coli genome. They saved amino acid sequences of the coding genes and that allowed the DNA to…

Bioinformatics coursework- report on IL2RG IL2RG (Interleukin 2 receptor, gamma) serves as a receptor for cytokines IL2, IL4, IL7, IL15 and IL21 (Brandt et al, 2007). The binding of IL2 regulates the activation, differentiation and proliferation of lymphatic cells through both positive and negative feedback loops. It prevents autoimmune diseases during the maturation of T cells, as well as playing a key role in cell-mediated immunity (Liao et al, 2011). The IL2RG gene is located on the…

manifestations such as vasculitis, rheumatoid lung disease, and Felty’s syndrome. Furthermore, joint damage is more rapid and progressive in patients with two alleles (Barton, 2015). Third, there are HLA DRB1 alleles which promote the citrullination of arginine residues. This process results in the formation of anti-CCP antibodies. According to Barton (2010), “The ACPA’s may be a path variable to explain the association of the shared epitope with RA susceptibility and/or severity” (p. 7).…

Genetic Disease Summary: Hemophilia A Hemophilia is an inherited bleeding disorder that slows down or prevents the blood clotting process. There are two main types of hemophilia: hemophilia A and hemophilia B. Hemophilia A, which is known as classic hemophilia, is caused by a mutation in the F8 gene. People with hemophilia often display excessive bleeding, including bleeding in the stool and urine, though it is usually painless. Joints are also heavily affected, causing swelling and decreased…

Summer is coming and what is the fruit that we enjoy eating in summer? If you thought of watermelon then you guessed it. It is really delicious and it is refreshing us in the hot days. Although there is one thing that many people are getting annoying when eating watermelons – the seeds. Many people are first removing the seeds then eating the watermelon and that takes some time to be done and that is the reason why the hate on the watermelon’s seeds is present. Also what many people do not know…