DNA ISOLATION INTRODUCTION: Deoxyribonucleic acid (DNA) isolation is an extraction process of DNA from various sources. Methods used to isolate DNA are dependent on the source, age, and size of the sample. Despite the wide variety of methods used, there are some similarities among them. In general, they aim to separate DNA present in the nucleus of the cell from other cellular components. Isolation of DNA is needed for genetic analysis, which is used for scientific, medical, or forensic purposes…
Abstract: A one dimensional variable range hopping type conduction is observed in Poly (3,4-ethylenedioxythiophene): polystyrenesulfonate (PEDOT : PSS) films and the mechanism behind enhancement in conductivity in 3% dimethyle sulfoxide (DMSO) doped PEDOT:PSS film is due to the phase segregation of PSS on the surface and reduction of energy barrier between the conducting grains. Enhanced conductivity of PEDOT:PSS films and transparency more than 80% in the visible region makes PEDOT:PSS films…
The purpose of this lab is to calculate a value for Avogadro’s number and compare it to the actual value for Avogadro’s number. Avogadro’s number will be determined by setting up an electrochemical cell to oxidize a copper electrode. The results of the amount of copper deposited and the average current used will be used to calculate a value for Avogadro’s number. Techniques used: In this experiment, different techniques will be used to collect the required data, such as taking the mass of…
Metal electrodeposition is the process of reduction reaction and crystallization of metal ions in an electrolytic cell according to the mechanism of electrolysis. Electrodeposition is one of the main methods for improving and enhancing the metal substrate property. Now, in order to overcome the defect of single electrodeposition with shortcomings of cracks, pores, pits, pocking and so on, the modern new techniques sprang up such as jet electrodeposition [1], ultrasonic vibration assisted…
The comet assay, also known as single-cell gel electrophoresis (SCGE), is a standard lab technique that can measure deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. This technique originated in 1984 with the purpose of quantifying the amount of DNA damage in a cell (3). Originally the test was run using neutral conditions, but this only allowed for detection of double stranded DNA breaks. In 1988 the technique was adapted to use alkaline conditions that made it more sensitive and…
and physics, substance that conducts electric current as a result of a dissociation into positively and negatively charged particles calledions, which migrate toward and ordinarily are discharged at the negative and positive terminals (cathode and anode) of an electric circuit, respectively.”Electrolyte. (2015). In Encyclopædia Britannica. Most electrolytes are acids, salts, and bases and they ionize…
DISCUSSION: In this investigation, the effect of electrolyte concentration, depth of electrode immersion in electrolyte, and distance between electrodes in electrolyte, on the current flow through copper electrodes were tested. Table 1 and Graph 1 display the results when increasing electrolyte concentration. Evident in Graph 1, the results contained scatter, and consisted of an outlier in the sample in which the ratio of NaCl: deionised water was 10:30mL. The outlier and scatter may have been…
Maple Syrup Urine Disease Hannah Gentry , 13SK , 8/21/2014 Maple syrup urine disease (MSUD) is a rare genetic disorder where an aminoacidopathy secondary to an enzyme defect in the catabolic pathway of the branched-chain alpha-keto acid dehydrogenase complex, which are required in order to metabolize certain amino acids in the human body. In other words, it is a metabolism disorder, where the infant is unable to break down the amino acids: leucine, isoleucine, and valine. Build up of these…
CHAPTER 1 INTRODUCTION Medical imaging is define as a techniques and processes used to produce the images of the entire or parts of the human body includes normal anatomy and physiology for clinical purposes. It includes a variety of modalities that gives focus to the needs of the patients. Among this are Ultrasonography (USG), Computed Tomography (CT), Computed Radiography (CR), Magnetic Resonance Imaging (MRI), Dental Imaging,…
productivity. Electrons can be generated using a hot wire, and directed through using an electron gun. The electron gun uses a circuit that is created by connecting a filament to a negative power supply, followed by accelerating the electrons to the anode plate. The electrons are coming from various angles. By creating a Wehnelt cylinder, the gun can become even more negative than electrons, forcing them into a converging pattern. The electrons are then passed through an aperture, escaping into…