Angiotensin-converting enzyme

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    Buffers Lab Report

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    buffer should be inert in the system being studied. For example, Tris buffer is unsuitable for some protein assays because it reacts with the assay components. Phosphate buffers contribute phosphate ions to a solution, which inhibit some types of enzyme reactions such as alkaline phosphatase. Tris-Borate-EDTA (TBE) buffer and Tris-Acetate-EDTA (TAE) buffers are most commonly used for DNA gel electrophoresis. However, because the borate reacts with hydroxyl group…

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    Amylase Lab Report

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    Assessment of enzyme producing efficiencies of endophytic fungi Extracellular enzymes assay was conducted to investigate the production of enzymes by the endophytic fungi Amylase activity Amylase enzyme was assessed by growing the fungi on glucose yeast extract peptone (GYP) agar medium (glucose- 1g, yeast extract- 0.1g, agar- 15 g, distilled water 1000mL and pH 6) containing 1 % soluble starch after 5 days incubation , the plates with fungal colony were flooded with 1% iodine and 2% potassium…

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    LO 2.1 I understand that free energy, the portion of a biological system’s that can perform work when temperature and pressure are uniform throughout the system, is used in systems to cause change. I also understand that empirical data proves all organisms require a constant energy output to maintain organization, to grow, and to reproduce. (Campbell 119-123) LO 2.2 I understand that although free energy is always required in living systems in order to maintain organization, to grow, and to…

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    Carbohydrates In Cars

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    Like cars, humans need fuel for energy. While cars use gas for energy, humans use nutrients for energy such as proteins, lipids, and carbohydrates. Proteins, lipids, and carbohydrates are the macronutrients the body need to function smoothly and properly.Out of the three macronutrients, carbohydrates is the main source for energy, it is the body's main source of fuel, and the nutrient use for brain function and organ operation. Unlike proteins and lipids,carbohydrates is easily digested and can…

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    Phenylketonuria Case Study

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    in phenylalanine (Phe) hydroxylase activity, due to mutations in the Phe hydroxylase (PAH) gene. Phenylalanine hydroxylase is responsible for converting Phe to Tyr and requires the cofactor tetrahydrobiopterin (BH4), molecular oxygen, and iron for its action (Blau et al., 2010). The reductant, BH4 is maintained in the reduced state by the NADH-dependent enzyme dihydropteridine reductase. Decreased PAH activity results in elevated Phe concentrations in blood and body tissue. As a result,…

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    Starch Digestion Lab

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    bath. We then repeated the same procedure using starch solution instead of maltose. When we did the test for digestion of starch. First, using separate medicine droppers, I transferred 3 ml of starch solution to a small beaker, then added a fresh enzyme solution consisting of a pinch of amylase in a 3ml of water and mixed thoroughly with a glass rod. Secondly, I waited for 1 min…

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    Simultaneous hydrolysis and fermentation (SHF) of sweet sorghum juice is a multi-step process and interactions between enzymes inside the yeast and the complex substrate. In addition, SHF also undergoesmechanisms by the product and other components inside the substrate. The process, interactions, and as well as inhibition mechanism of SHF are not fully understood. A modified kinetics model based on those reported by Philippidis [24] and Shadbahr [17] were used in this study to quantify the…

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    Enzyme Catalysis Lab

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    Enzyme catalysis can be a very confusing and long process, but in easier terms can just be explained by the fact that enzymes break down molecules into smaller pieces. The objective of this lab is to observe how quickly the enzyme catalase breaks down H2O2, and what long periods of contact between the two do to that process. We used seven different cups for seven different lengths of time, and in every cup we mixed 10 mL of 1.5% H2O2, 1 mL of distilled water, and 1 mL of catalase. We then…

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    Lead Identification

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    Lead Identification (LO): Lead compounds bind to target receptors, and shows potentially therapeutic pharmacological activity, and may contain similar structures and functional groups. Lead compounds are used as a foundation for drug development and may be modified structurally for greater pharmacodynamic efficiency and potency.1 One source of determining lead compounds is by assaying libraries through HTS. Researchers analyze the drug’s ADME properties (Absorption, Distribution, Metabolism, and…

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    Number6 Nd Research Paper

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    Number6. This is known as the ‘goddess’. Start with hands straight over your head in mountain position. Then spread your legs apart and bend them until you can feel your thighs being parallel to the ground. Spread your hands on the both side of your head and lift them up at a ninety- degree angle. Hold onto this position for at least 10 seconds for five rounds. Number7. Stand straight. Now, lean on the left side with your left elbow touching the left knee. Swing your right hand over your head to…

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