This test is performed on organisms to see if it has the enzymes to reduce the Nitrate to Nitrite. To run the test, it will require using two colorless chemicals (so make sure you are paying attention) that will either change the color of the organism by the interaction of the new chemicals with the enzymes found in the unknown organism. So, if the organism has the capability to reduce the nitrate to nitrite the solution in the test tube will turn red almost immediately and the organism is…
The lab that is being preformed is about digestive enzymes. Enzymes are protein molecules produced by living organisms that speed up a specific chemical reaction in the body. There are also digestive enzymes which digest our food. Our bodies make digestive enzymes in the pancreas, stomach, small intestine, and mouth. Digestive enzymes break down the food we eat into nutrients so that our bodies can absorb them. There are different digestive enzymes that digest certain macromolecules such as…
from the cells, subsequently followed by excretion into bile or urine1. The metabolic enzymes in charge of this process are traditionally organized into two separate categories; phase I (oxidation, reduction, or hydrolysis) and phase II (conjugation) enzymes1,2. During phase I, polar groups are added to the molecules through oxidation, reduction, and hydrolysis, mediated generally through cytochrome p450 enzymes. Phase II…
Interpretation 1. Theoretical Lab Discussion This lab occurs due to enzyme kinetics, and the kinetics molecular theory. Enzyme kinetics is the study of chemical reactions that are catalysed by enzymes, and during the experiments, the reaction rate was measured by the effects of the varying conditions the substrate was in. Influence of Catalase Concentration The higher the concentration of the enzyme catalase, the function of the enzyme occurs better. As the number of disks increased, the…
syrup since it is much more effective. Pineapple contains an enzyme called Bromelain which is effective in fighting infections and bacteria. This enzyme makes pineapple juice a powerful anti-inflammatory agent. Half the amount of vitamin C that you need per day Only one glass of pineapple juice will provide your body with half of the daily dose of vitamin C that you need. Vitamin C also enables your organism to metabolize the enzymes used to ménage energy and regulate the function of the…
oxidation, as well as break it down by converting it to water, or using the hydrogen peroxide to oxidize other organic compounds. They also produce energy by metabolizing fatty acids (Cooper, 2000). Peroxisomes execute various metabolic functions and create hydrogen peroxide as a byproduct from the process of oxidation. However, since the hydrogen peroxide created by the peroxisome is toxic, the organelle also produces an enzyme called “catalase”. The catalase enzyme breaks down the hydrogen…
Hemoglobin has a peroxidase-like activity, meaning that it will break down hydrogen peroxide, or other organic hydroperoxides. This makes it useful in forensics, since you can test for the presence of hemoglobin simply by dropping hydrogen peroxide on it. Of course, there are a lot of things that have peroxidases in them, so this is a presumptive test for the presence of blood. Blood cells are not immortal; each cell wears out in about four months, and will disintegrate in the blood stream,…
special proteins called enzymes. Enzymes are long chains of amino acids that have taken a very specific three-dimensional shape called a globular shape. This specific shape contains an area that is designed to match a specific molecule. The substance that an enzyme operates on is known as its substrate. Almost all metabolic reactions in organisms are catalyzed by enzymes. Many of these reactions occur in specific sequences and are called metabolic or biochemical pathways. Enzymes can be…
Enzymes Two major factors influence how effective an enzyme is by promoting the rate of reaction. Each enzyme has a specific temperature that allows it to perform the most work. If an enzyme is placed in an environment under its optimal functioning temperature, the speed of the reaction will slow. Reason being, the molecules of the reactants reduce in energy from the low temperature, less movement means less reactions with the enzymes. At higher temperatures, molecules within an enzyme increase…
Dissolution test will be carried out in a simulated gastric fluid (SGF) using hydrochloride acid or a neutral buffer phosphate solution. The simulated gastric fluid without enzyme (SGF) at pH 1.2 and pH 3 containing sodium chloride and hydrochloric acid in 900ml deionized water will be prepared. Thus, the pH will be adjusted using hydrochloride(Ghazal et al., 2015). The simulated gastric media containing milk (whole milk, skimmed milk) will be prepared using an equal volume of milk and (SGF) pH…