base linear pairs. Three common restriction enzymes: EcoRI, HindIII and PstI are used to separate the DNA fragments. The task at hand was to separate the fragments of DNA. Separation was to be done based upon size using gel electrophoresis. Then compare the DNA fragment of known sizes by millimeter or molecular weights, or standards to the DNA restriction fragments. Each enzyme cuts at a different place in the lambda DNA. This happens because each enzyme has a different restriction…
4.1 Maintenance and sub-culturing of endophytic fungi The cultures were procured from already maintained repository in laboratory. The maintenance of the cultures involved preparation of Potato dextrose agar (PDA) plates, sub culturing of cultures and long term preservation. 4.1.1 Preparation of PDA (Potato dextrose agar) plates 39g of PDA was dissolved in 1000 ml double distilled water, stirred to mix properly and was transferred into 250 ml Erlenmeyer flasks and autoclaved at 121˚C at 15…
1.4 Effect of Microcrystalline Cellulose (MCC)Type Formula F12 was utilized to study the effect of changing the type of MCC(Avecil PH-101 ) as a carrier material instead of (Avecil PH-102) in F8 on the dissolution behavior of the prepared Zolmitriptan liquisolid ODTs. Although the DT of formula F12 (79 sec.) was greater than F8 (48 sec.) as in Table(18),the dissolution behavior of the formula F12 was faster than F8 as in Table (18) and Figure (48), this difference was non…
Since it is large you can imagijne that it will have a lot of functions such as, it stores vital energy and nutrients, it manufactures proteins and also manufacvtures enzymes that is necessary for good health, it protects the body from diseases, it also breaks down or metabolizes and it removes harmful toxins, such as alcohol, from the body. Since the liver is the major organ for removeing toxins such as alcohol, a small…
Table 1: Experimental data for standardization of iodine solution. Calculation of standardization factor Standardization factor = (mass of vitamin C titrated) / (Volume of iodine solution used) = (125g) / (41.3ml) = 3.03 mg vitamin C/ml iodine solution Experiment B: Vitamin C titration using fresh fruit sample to determine the content The tabulated experimental data for this experiment is shown below (Table 2). In this experiment, the amount of vitamin C was calculated by multiplying…
Results The results obtained were statistically analyzed using the Fishers exact test. Table 1: Comparison of microbial growth between different groups Sodium Hypochloride Total Fishers exact test Endovac Laser Control p-value No Growth 5(50.0%) 8(80.0%) 0 15(37.5%) <0.001* <103 5(50.0%) 2(20.0%) 0 7(17.5%) 103 – 108 0 0 0 8(20.0%) >108 0 0 10(100.0%) 10(25.0%) Total 10 10 10 40(100.0%) *p<0.05 statistically significant p>0.05 non significant, NS Amongst the 10…
Bessara of Fava Bean from Morocco Serving size: 1 Servings per Recipe: 4 Calories: 357 Cooking Time: 20 minutes Ingredients: 1. Garlic- 2 cloves, slightly crushed 2. Split Fava Beans- 2 cups 3. Vegetable oil- 2 tbsp. 4. Water-3 cups 5. Cumin Powder- 2 teaspoons 6. Tahini- 2 teaspoons 7. Harissa- 1 teaspoon 8. Lemon- squeezed juice and zest separately 9. Salt- 2 teaspoon 10. Paprika to taste 11. Olive oil- 1 tablespoon Nutritional Information: 1. Total Fat – 24.6g 2. Sodium- 335.6g 3. Total…
Phase Solubility results The phase-solubility diagram (figure 1) obtained was classified as AL type(20) because the apparent solubility of ISH increased linearly with β-CD concentration over the entire concentration range studies, thereby suggesting the formation of a water soluble complex. A slope value of less than one indicated that an inclusion complex in a molar stoichiometric ratio of 1:1 was formed between the drug and β-CD in solution. The stability constant (718.58 M–1), computed from…
Boris Kozarski Lab partner: Donovan Ortiz 9/23/16 Bio 118: A-65 GTA: Mian Li, UGTA: Shelby Schaffer Intro: Enzymes are important biological molecules that help catalyze numerous reactions in the body. They are an arrangement of proteins designed to serve very specific roles. Enzymes contain active sites that substrates bind to. The enzymes then help orient those substrates so they achieve ideal reaction conditions. As a result, substrates are broken down much faster than they would on their own…
ABSTRACT: This study was carried out to evaluate the effect of pretreatments prior different drying methods and temperatures on compositional quality of the dried mushroom. Oyster mushroom (Pleurotus ostreatus) was cultivated on rice substrate and collected samples were pretreated (steeped or blanched) by different solutions (0.1% sodium metabisulfite and 0.1 and 0.5% citric acid) and dried using oven at 50°C and 60°C, sun-drying at 30-35°C and microwave drying at 2.45 GHz until reached to…