Angiotensin-converting enzyme

Background: Isopods, or what are often called pill bugs, are crustaceans. Isopods live in damp environments. They can be found under rocks, logs and in soil. In this lab, 10 isopods were given a choice between salt water or regular water chambers to test their preference. The results were recorded every 30 seconds over a period of 10 minutes. Problem: It is known that isopods have a preference for moisture.This lab will specifically test whether or not an additive will affect a pill bug's…

The second part involves finding the best speed for optimization. Eight different tests will be performed. Four in the first part and four in the second part. Each test will involve about 800 mL of the sample. In the first part, four tests were created of alum concentrations of 0, 5, 20, and 40 mg/L. All were subjected to the stirring apparatus at the same speed of 30 rpm for 15 minutes to allow for flocculation. Initial turbidities were taken before starting the mixing. After being mixed, they…

by denaturing the non-recombinant E.Coli proteins. The Second step was much more beneficial to the total purification but the combination of the two steps achieved an even better purification of 4.6 fold. From this assay we can see that the correct enzyme has been successfully purified. Considering the SDS-PAGE results we can confidently say that the purification steps were effective at removing a large proportion of E.Coli proteins. However, not all E.Coli proteins have been removed as shown by…

In our hypothesis we predicted that if borax is added to a laminated rubber-plastic composite it will strengthen it and the end product will have a higher puncture strength. From the data that we got by testing our plastic, it is maybe supported, but I cannot confidently say it is true judged by how weak our all data inclination is. The overall higher puncture strength is when we added 12 mL of borax to our laminated plastic. It may have been because borax is sticky substance and can cause the…

Rabia Iqbal Biol 395-1005 lab 01/31/2015 Broadford assay Absorbance Figure 1. The concentration and absorbance of Bradford assay. Four samples containing Bovine serum albumin, diluent, and Bradford. At 0.25 mg/ml concentration the absorbance was 0.109 nm and the relation between absorbance and the concentration held constant throughout except at the 0.50 mg/ml when the concentration absorbance was 0.186 nm. Figure showed that as the concentration is increased the absorbance elevates also. All…

First Experiment- One lab partner should prepare a test tube that contains 10 mL of the lactose solution. Make sure to label the tube as being Tube One. Another lab partner should prepare a test tube with 10 mL of the lactose solution and 1 mL of the lactase solution. Take caution in measuring the solutions. Label the tube with lactase and lactose as being Tube Two and cut off an appropriate amount of paraffin wax paper to cover the opening of the tube. Press the paper tightly to the tub and…

Aim: The aim of this investigation is to determine the effect of water temperature on the time it takes for an aspirin tablet to dissolve. Hypothesis: The boiling water will have the quickest time to dissolve the aspirin tablet. This is because its molecules have more energy and are vibrating more rapidly than the cold water. Cold tap water will have the slowest time to dissolve the aspirin tablet because it has the least kinetic energy. Why? Apparatus: Equipment: Image Kenwood Kettle Pyrex…

The DE2-115 peripherals lab results were quite interesting, both numerically and visually. In part one of the lab, the results pertained to the largest number a bus could hold. For example, it was calculated that a bus of [17:0] could hold any number up to the limit of 262143 using the equation (1). Also, when the value of X in Figure 5 was changed a certain number of lights would begin to blink on the LEDs above the switches. As the value of X increased the number of the switch that would…

For part 2 of the experiment (please refer to next page for Figure 3), Figure 3 shows a graph comparing the absorbance readings of the Glutamate-pyruvate aminotransferase serum with the time in minutes. A similar rate was predicted to occur for both the assays done. This would be characterized by the readings showing two parallel lines on the graph. However, the two first readings did not demonstrate this. This could possibly be due to different and insufficient amounts of NADH+ being added to…

to increase the amount of surface area which is available to absorb nutrients. Epithelial tissue it covers the inner and outer surface of a stomach to protect against acid such as hydrochloric acid. The epithelial tissue secretes digestive fluids, enzymes and acid. It acts as a barrier and an absorbingliner. An organ is a group of different tissues that work together to perform a certain function. The stomach is a muscular sac which lies between the oesophagus and small intestine. The stomach…