Angiotensin-converting enzyme

Eosin methyl blue (EMB) agar and MacConkey broth w/neutral red, HiMedia Laboratories Pvt. Ltd. India for coliform; Plate count agar, HiMedia Laboratories Pvt. Ltd. India for TPC; Potato dextrose agar, HiMedia Laboratories Pvt. Ltd. India for yeast & mold testing. Methyl Red Indicator, MERCK, India; Iodin, Qualigens, India; Methyl Orange, Qualigens, India; Ferroin Indicator Sol, MERCK, India; Glycerol, Qualigens, India; Methylene Blue , Qualigens, India; Oil emersion, MERK, India; Christal…

Km and Vmax determination of glucocerebrosidase towards p-NPG The Michaelis constant (Km) is a measure of the affinity of the enzyme towards the substrate, with smaller values representing greater affinity. Km and the maximum rate (Vmax) of leukocyte GCase were obtained through the Lineweaver-Burk plot (Figure 4) with artificial substrate p-NPG in concentrations from 0.71 mM to2.50 mM. Km and Vmax values for leukocyte GCase using p-NPG as substrate was found to be 12.6 mM and 333 U/mg…

Introduction We are testing the affect of substances on bread mould growth. The question we’re hoping to carry out is what antiseptic and cleaning substances would have on the effect of the growth of bread mould. And whether it would inhibit the mould, increase the growth of the mould or have no effect at all. Hypothesises: it is expected that antiseptic substances such as Jik, Mr Muscle and Handy Andy will inhibit the growth of fungi, as these antiseptic substances will effectively stop the…

Normal standards for specific gravity are as typically as follows: 1.000 to 1.030 is a normal specific gravity, 1.001 after drinking excessive amounts of water, more than 1.030 after not drinking very many fluids and concentrated after receiving an antidiuretic hormone (ADH). Max’s urine concentration varied in concentration each time he was tested. The first test showed that his urine was a pale yellow which was an indication that he was hydrated. The second test showed the concentration to be…

Background Research: Cellular respiration occurs in all living organisms. ATP is a molecule that is made from food. Yeast uses cellular respiration as well. While the process of cellular respiration occurs in yeast, carbon dioxide is produced. Stephanie Lamot of Northeastern Illinois University conducted an experiment on how different types of sugar have an effect on yeast. Her conclusions were that the more yeast added to sugar, the faster the production of CO2 is. Hypothesis: The rate…

Effect of temperature Results In this experiment, results from two Procambarus propinquus (crayfish) were collected to observe the difference in oxygen consumption at different acclimated temperatures. These cray fish were collected from Medway Creek in London, and acclimated to their new environment and temperatures. The crayfish were exposed to the cold environment having an average temperature of 7° or warm having an average of 22°. An unpaired, two-tailed t test was performed to determine if…

The objective of this experiment in Lab #2 was to identify an unknown substance using different chemical tests to determine what organic molecules it contains. These tests are referred to as the Benedict’s, Lugol’s, Biuret, and Sudan IV tests and are each used to identify an organic compound. For instance, Benedict’s checks for reducing sugars, Lugol’s for starch, Biuret for protein, and Sudan IV for lipids. When testing the unknown with each reagent, the substance only tested positive for…

During this lab, my main goal was to discover exactly how much phosphorus (or phosphate) is in the composition of plant food, or in the case of the Guided Inquiry portion of the lab, in two different brands of plant food. If I put it in the most concise way possible, to do this, I carried out quite a few processes that caused the components of the plant food to separate from each other. Basically, I got the phosphorus, along with some other substances, to form as a precipitate. Next, through a…

Enzymes are proteins that are a efficient catalyst for a biochemical reaction. It’s responsible to speed up reaction by having an alternative pathway of low active energy. In a simple cell it can be found 2000 different enzymes, each one of these have the ability on catalyzing a different reaction. Each of the enzyme found are responsible on having a reaction occurring in the cell. In this certain reaction in the cell, the cell will tend to make poisonous chemical that could be harmful to the…

conventionally spherical or oval shaped, 0.5µm in diameter and around 0.5-5µm in length. The internal lumen of a lysosome contains enzymes that catabolically break down biological macromolecules via hydrolysis reactions (insertion of a water molecule that breaks intramolecular bonds). Enzymes include; proteases, ribonucleases, deoxyribonucleases – all defined as hydrolases. Such enzymes are synthesised at ribosomes found on endoplasmic reticulum, and are packaged into vesicles that bud from the…