Angiotensin-converting enzyme

Practical 3: Enzyme Activity (2.5) Research Question: How does the change of pH affect the rate of decomposition of hydrogen peroxide on the enzyme, catalase? Background Information: Enzymes are globular proteins, which are synthesised by cells in order to perform specific functions. These act as catalysts in chemical reactions and are considered a major factor in an organism as these reaction rates are required to be shortened in order to support life. Catalase is one of the most…

I. PURPOSE The purpose of this lab was to observe the physical properties of different samples of matter and to use your observations and knowledge of chemistry to classify each sample as an element, a compound or a mixture. II. MATERIALS 10 beakers containing different samples of matter III. PROCEDURE First off, put on a laboratory apron. Then, take a few minutes at each lab station and record three physical property observations of each sample of matter. Record the three physical property…

Immunocytochemistry (ICC), which this lab is based on, is commonly used as a laboratory technique to anatomically observe the location of a specific protein or antigen in cells by the use of a specific primary antibody that binds to it. A staining reagent or chromagen is used to help detect certain proteins used in this experiment, called 3,3'-Diaminobenzidine (DAB). 3,3'-Diaminobenzidine (DAB) DAB, a derivative of benzene, is an organic compound, which is both chemically and thermodynamically…

leucine to proline at amino acid position 1544(Ashcroft, 2005). This mutation simply reduce the activation of the channel by stimulatory nucleotide MgADP, as the NBDs sensitivity to this nucleotide is greatly/entirely lost. The failure of the channel activation means the open state of the channel pore is greatly reduced/blocked. This occurs as the inhibitory nucleotide (ATP) would have higher affinity for the channel than the activating nucleotide (MgADP). Closure of the K+ channel leads to…

industrial processes due to their natural metabolic capabilities are known as biotechnology. They are used a several ways such as manufacturing of foods, cosmetic products and production of antibiotics, probiotics, drugs, vaccines, insecticides, and enzymes. In addition, they are also being widely used in the genetic engineering. Nowadays, bacteria are commonly used in the food industry for the manufacture of dairy products such as cheese, milk and yogurt and also in the fermentation of alcohol.…

In our experiment we evaluated which liquid yeast would produce gas the fastest by measuring how long it took for certain liquids to inflate a balloon. We hypothesized that if the yeast and sugar were placed in a warm,sugary environment like apple juice, then the yeast will produce more CO2 and cause the balloon to inflate faster. The control was the room temperature Throughout the experiment the type of balloons, size of the erlenmeyer flasks, amount of yeast, volume of each liquid, and the…

(Vmax) and the inhibition of alkaline phosphate. In order to accomplish these goals, 5 samples were used. Each sample contained different volumes of 0.2 m MPNPP (p-nitrophenylphosphate) and 0.2 M Tris-Hcl at a pH of 8.0. To each sample 0.2 mL of the enzyme studied (Alkaline Phosphatase) were added upon insertion on the spectrophotometer apparatus. With intervals of 20 seconds their absorbance at a wavelength of 410 nm was recorded at time frame of 2 minutes for each solution. The initial…

and less in the honey. According to Menezes et al. (2013), yeasts have the important potential roles for the meliponine colonies as similar as the bacterial roles. For example, some bacteria undergo the metabolic conversion process by secreting enzymes that breakdown substances from stored food into a simple form for conservation (Gilliam, 1990). The participation of bacteria and yeasts in a lactic acid fermentation has often been postulated for the conversion of pollen into bee bread (Haydak,…

Introduction Homeostasis is the state of a stable internal cell environment. If the cell is not in a state of homeostasis, then the cell will not be able to live and function properly. The plasma membrane is selectively permeable and controls what molecules enter and exit the cell. The plasma membrane keeps the environment inside the cell relatively stable which is the state of homeostasis. The goal of this investigation is to see if the size of the cell is affected when the cells are put in…

The purpose of this lab is to find the optimal pH of the enzyme peroxidase, which is found in plant and human cells as a byproduct of photorespiration, photosynthesis or cellular respiration. This is significant because an enzyme operating in nonoptimal pH could have negative effects on the cell, and could pose a health threat to the organism. Enzymes proteins that speed up chemical reactions by lowering the activation energy. They do this by acting as a template for substrate orientation,…