Analytical engine

1. Abstract In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase,…

High performance liquid chromatography (HPLC) is a separation technique utilizing differences in distribution of compounds in two phases; called the stationary phase and the mobile phase. The stationary phase designates a thin layer created on the surface of fine particles and the mobile phase designates the liquid flowing over the particles. Under a certain dynamic condition, each component in a sample has a different distribution equilibrium depending upon the solubility in the phases and or…

The chromatography was performed on an ACQUITY TM UPLC system (Waters Corp., Milford, MA, USA). The UPLC system included quaternary solvent manager, a binary pump, degasser, autosampler with an injection loop of 10 μL and a column heater-cooler. The separation was performed on Acquity UPLC BEH TM C18 column (50 × 2.1 mm, i.d., 1.7 μm, Waters, USA) maintained at 40 °C. a mobile phase of acetonitrile containing 0.1% formic acid and aqueous 5 mM ammonium acetate buffer containing 0.1% formic acid…

A Jungian Analysis of Ursula Hegi’s Stones from the River “We cannot change anything until we accept it. Condemnation does not liberate, it oppresses.” - C.G. Jung. Carl Jung’s theories are profoundly influential to modern psychiatry; they allow us to analyze stories, characters, and writings from the innermost layer. Although Carl Jung did extensive research on the human psyche, perhaps some of his most interesting theories were on the nature of how human beings write and tell stories,…

When a light is shine to a person, people can him or her clearly. You cannot hide anything, but look behind and a shadow is standing right behind. The shadow self or known in psychology as the personal shadow is an assertion that everybody has another personality deep within them. First formulated by Carl Jung. It has been developed by his followers, some of them are Connie Zweig and Jeremiah Abrams. The shadow self is a personality hidden to the outside world, either by design of the person or…

have the potential to bind to other proteins. Once this red colored species bind to a protein the bound dye molecule transforms to blue species, and this color change is the one measure by the spectrophotometrical. The assay is a spectroscopy analytical technique based upon the absorbance maximum for an acidic solution of Coomassie Brilliant Blue. From the assay is observed the maximum absorbance for an acidic solution of Coomassie Brilliant Blue G-250 mostly shifts from 465 to 595 nm upon…

When I initially read this essay prompt, I figured it would be a simple task to answer it effectively. I mean, what could be easier than writing about yourself, right? However, as I contemplated what my answer would be, I realized that it is not quite as simple as I had first thought. There are many aspects of “who” a person is. Should I answer it in a personality context or should I be focusing on my likes and dislikes? Should I place a heavy emphasis on my spiritual journey and viewpoints…

Introduction Water hardness is defined as the sum of the concentrations of Ca2+ and Mg2+ in a water sample which is measured by amount of CaCO3. Typically, water hardness indicates the water quality, as hard water has greater concentrations of Ca2+ and Mg2+ and soft water has proportionally less concentrations of the aforementioned ions.1 Water that contains Ca2+ and Mg2+ concentrations above 100 milligrams per liter (mg/L) is categorized as hard while soft water contains ion concentrations…

Table 1 Composition of Lansoprazole microparticles Formulation code Amount of drug(mg) Amount of polymer chitosan (mg) Drug polymer Ratio Tween 80 (%w/v) F1 100 100 1:1 1.25 F2 100 200 1:2 1.25 F3 100 300 1:3 1.25 F4 100 400 1:4 1.25 F5 100 500 1:5 1.25 F6 100 600 1:6 1.25 Characterization of Lansoprazole microparticle The microparticle will be characterized for following parameters: Percentage yield: After complete drying, the drug loaded microparticle was collected and weighed accurately…

compound with high reproducibility. The materials for constructing a HPLC column are inert to the analytes such as polished stainless steel, glass and PEEK polymers. There are mainly four types of columns used in HPLC which are high performance analytical column, preparation column, capillary column and…