Analytical chemistry

1. Abstract In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase,…

High performance liquid chromatography (HPLC) is a separation technique utilizing differences in distribution of compounds in two phases; called the stationary phase and the mobile phase. The stationary phase designates a thin layer created on the surface of fine particles and the mobile phase designates the liquid flowing over the particles. Under a certain dynamic condition, each component in a sample has a different distribution equilibrium depending upon the solubility in the phases and or…

The invention relates to formulation of Ketorolac Tromethamine and Phenylephrine Hydrochloride immediate release tablet having less disintegration time. HPLC method was developed and validated for determination of content of Ketorolac Tromethamine and Phenylephrine Hydrochloride in immediate release tablet. UV spectrophotometric method was developed and validated to study drug release profile of Ketorolac Tromethamine and Phenylephrine Hydrochloride in immediate release tablet. Background:…

Analytical Method Validation of Sylimarin The λmax of Sylimarin in ethyl alcohol and PBS was found to be 286 nm and 322nm, respectively. The drug follows linearity in the concentration range 2–20 μg/ml with a correlation coefficient value of 0.998 and 0.999 for in ethyl alcohol and PBS, respectively. The accuracy of the method was checked by recovery. The % recovery was found to be in the range of 99.54– 100.98% in ethyl alcohol and 98.94– 101.4% in PBS, respectively. The precision of the…

Ali Youssef CHM 111-0A7L Mitra Jahangeri Paper Chromatography Lab Discussion The results of the Paper Chromatography process seem to have aligned with what was expected. When the capillary action on the paper had completed and the chromatograph was finished, the distance that each cation moved (D), as well as the distance that the solvent moved (L), could be properly measured. The distance that the solvent moved ended up being 7.05cm, a number that would represent the constant variable L in…

Egypt The Egyptian outlook on perfumes could be derived from their lifestyle learned from the records, the objects found in connection to death and afterlife and mummified remains. The ancient Egyptians were concerned with the odours of everyday life and death. Dated to 1550 BCE, the Ebers papyrus gives us a wealth of knowledge on Egyptian medical preparations for ailments, perfumed anointing oils, and incense plants. As we move back through time, as early as the third millennium BCE, it…

4.2.3.5. Antioxidant assays in gastric tissue homogenate 4.2.3.5.1. Catalase activity Catalase activity in the gastric tissue was measured according to the procedures of Sinha (1972). One ml of homogenate solution was taken and mixed with 5 ml of phosphate buffer of pH 7.4. To this 4 ml of 0.2 M H2O2 was added and time noted. Exactly after 180 seconds of adding H2O2, a set of 1ml of reaction mixture from the above was taken and 2 ml dichromate acetic acid was added. It was then kept in a boiling…

Gas chromatography (like any other chromatography) is a separation technique in which the mobile phase is a gas. This technique is applied for the separation of volatile substances. This method separates a mixture into its components and is used to measure the concentration as well as it identifies the components. GC creates a time separation rather than physical separation which means that the components are separated in time. After they are detected, a chromatogram is generated. Each peak in…

Science that I decided that my passion for science would be satisfied by a Neuroscience degree course. Chemistry has long been my favourite subject at school and the topic which interests me most is organic chemistry, especially aromatic compounds. I entered the Cambridge Chemistry Challenge and succeeded in achieving a silver award. I also undertook a period of work experience in the Chemistry Department at Manchester Metropolitan University, working in the laboratories with technicians…

In Lisel Mueller’s poem “Reading the Brothers Grimm to Jenny,” the narrator details the relationship that he or she has with Jenny. Jenny is a character who is facing her own internal conflicts, but displays innocence and truthfulness to the narrator. As a result of this display, the narrator is conflicted about telling fairytales to Jenny. Moreover, the narrator is concerned with the high qualities and ideas that she attributes to people who are not worthy, who the narrator deems unworthy. To…