Analytical chemistry

Chromatography is a process used to separate molecules on the basis of chemical or physical properties, such as molecular mass, charge, or hydrophobicity. (Ninfa 121) There are many types of chromatography processes that can be used in different experiments and scenarios. These chromatography processes on include Gel filtration, ion exchange, and high pressure liquid chromatography. However, in this laboratory session the process of affinity chromatography is utilized to purify a His-tagged…

air-dried for 2 days at room temperature. The collected leaves (Fig. 1) were freeze-dried immediately after sampling (harvest dates:30th March, 10th April, 28th April, and 12th May, 2013) and stored at –40 ºC prior to analysis. [Insert Figure 1] Analytical grade water, acetonitrile, and TFA were purchased for UPLC analysis from J.T. Baker (Phillipsburg, NJ, USA). For quantitative analysis, phenolic compounds used for the standards were isolated from barley leaves, as reported in earlier…

Introduction Chromatographic process is a separation technique which has to do with the mass transfer of test sample between a stationary and mobile phase. HPLC (High Performace Liqud Chromatography) is a vast system which involves the use of a mobile and stationary phase to separate materials. The stationary phase is usually a column packed with solids (usually silica gel) while the mobile phase is usually a solvent or a mixture of various solvents. The mobile phase usually, is the carrier of…

Thin Layer chromatography was also use to separate the mixture of fluorene and fluorenone. Thin layer chromatography is a useful separation method when the components of a mixture are in question. Analyzing what the mixture consist of can be efficiently determine by TLC by calculating Rf and comparing these values. For the separation and isolation of a mixtures components, this method is extensive and not as efficient at column chromatography. This conclusion is represented in the data gathered.…

Protein Separation Using Gel Filtration Chromatography Introduction: Chromatography techniques are one of the most useful methods use for the isolation or separation of macromolecule from a mixture. The most common used chromatography techniques are thin -layer chromatography (TLC), column chromatography, etc. The purpose of this lab exercise is using the gel filtration chromatography to separate different proteins from a mixture based on their size. Also, collecting elutes that run through…

ABSTRACT: In this experiment, we determined the relative polarity of substances using the principles of chromatography. Three substances were tested using a thin layer chromatography. The column chromatography tested a mixture of two substance which were separated as a result of the chromatography. The results for both the thin layer and column chromatography supported the hypothesis. Therefore, chromatography is an effective tool that can be used to determine relative polarities. INTRODUCTION:…

Table 1 Composition of Lansoprazole microparticles Formulation code Amount of drug(mg) Amount of polymer chitosan (mg) Drug polymer Ratio Tween 80 (%w/v) F1 100 100 1:1 1.25 F2 100 200 1:2 1.25 F3 100 300 1:3 1.25 F4 100 400 1:4 1.25 F5 100 500 1:5 1.25 F6 100 600 1:6 1.25 Characterization of Lansoprazole microparticle The microparticle will be characterized for following parameters: Percentage yield: After complete drying, the drug loaded microparticle was collected and weighed accurately…

like molecular weight, density, shape, etc. If it is used for the separation of one type of materials from others is termed as preparative centrifugation and when used for measurement of physical properties of macromolecules, then it is known as analytical centrifugation. Principle of centrifugation: Particles suspended in a solution are pulled downwards by Earth’s gravitational force. In a solution, particles with higher mass…

1. It is necessary to list the experimental conditions when reporting a chromatogram because it will allow universal comparison. In the event that you want to compare your results with a colleague, the factors that can affect the results are listed and taken into consideration to get an accurate comparison. 2. The type of column used is listed, this column contains the stationary phase and the mobile phase is pumped through the column under pressure. The dimensions of the column are listed as…

Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of quetiapine fumarate from marketed bulk tablets. The active ingredient of quetiapine fumarate separation achieved with C18 column using the methanol water mobile phase in the ratio of 30:70 (v/v). The active ingredient of the drug content quantify with UV detector at 359 nm. The retention time of quetiapine fumarate is 5.27 min. A good…