through it. Bacteria that have a flagella are known as motile and the bacteria that do not are non-motile. The motility test is conducted by flaming an inoculation needle. After grazing the top of a culture, then placing the needle straight down into a tube of the motility media and lifting the needle straight out. Triphenyltetrazolium chloride (TTC) turns the bacteria to a red color making it easier to see if the results are motile or non-motile. If the bacteria form a straight line it is non-motile, however if the bacteria is spread throughout the tube it is classified as being motile.
Species like bacteria can either live with or without oxygen. If the bacteria need oxygen to survive, the bacteria are known as obligate aerobic. Obligate anaerobic mean the bacteria do not use oxygen and the presence of oxygen kills them. Facultative anaerobes can survive with or without oxygen. To differentiate between the three options of the species an oxygen requirement test is performed. The motility media must be melted at 60˚C. When the media cools, use an inoculated loop to get a sample of the culture. Then, dip the sample into the media and mix it throughout the tube. After the results have finished the obligate anaerobe will be only within the top two centimeters of the media agar. The obligate anaerobe will be at the bottom two centimeters due to the absence of oxygen. If the bacteria is throughout the tube it is a facultative anaerobe.
The lactose fermentation test is used to…
Methane is the final product of Anaerobic Digestion. A mixture of methane and carbon dioxide is called a biogas and it can be used for heating and can be used as a natural gas. Anaerobic treatment systems are divided into “high rate” systems involving biomass retention and “low rate” systems without biomass retention. High rate systems are characterized by a relatively short hydraulic retention time but long sludge retention time and can be used to treat many types of wastewater. Low rate…
decomposable objects. I believe that learning more about biodegradable, compostable, and decomposable objects may help to explain the law of conservation of mass.
There are many types of decomposition. There is anaerobic…
A review of Methanoculleus: a hydrogenotrophic methanogens frequently observed in anaerobic habitats
Keywords: Methanoculleus, hydrogenotrophic pathway, methane production
Methane is an important greenhouse gas due to its capability of retaining heat and its release from many nature sources, including wetlands, fresh water or sea water sediments, and animal manure, etc. There are two main means of atmospheric methane production, biogenic and abiogenic. Since 70%-80% of…
Importance of Fitness: Aerobic & Anaerobic Training in soccer
Florida Atlantic University
HSC2100: Health, Fitness for Life
November 6, 2014
Utilizing the research of Stewart Flaherty (2013), Sarah R (2010) and Beth Mervyn, (1991), this paper reflects understanding of how aerobic and anaerobic training influences the maximum distance a soccer player is able to cover in a soccer game. Also identified, are the possible…
II. Open dump and environmental problems
III. Early landfills
IV. Modern landfills
V. Mariannhill Landfill, South Africa
Municipal Solid Waste (MSW), also known as refuse, trash, rubbish or garbage, refers to a type of waste that comprises of items that are discarded by the public every day. The management of this waste has evolved over the years. This forms the basis of this essay.
Open dump and environmental problems
Open dumps are…
Because Germany, Austria, Belgium, and other European countries have a relatively perfect waste source separation system and mechanical sorting facilities, mechanical-anaerobic digestion technology was first applied in 1984 and now has been widely used to handle food waste, fruit and vegetable garbage, yard waste, and organic waste by mechanical sorting.
10774BMunicipal waste collection started late in China, and the Ministry of Construction identified Beijing, Shanghai, Guangzhou, Shenzhen,…
You asked the following questions: “A new is building being built that is adjacent to an existing building. The existing building has a wet sprinkler system and the new building will have a wet sprinkler system with a separate riser. Both risers feed off the same looped fire line that circles the property. The AHJ wants to consider it one structure since the buildings are adjacent. The issues in question are: 1) Is there anything in NFPA 13 that restricts a building to having only one riser…
presented in peer reviewed journals and government sponsored reports in the hope that these findings are based more on sound research and systematic analysis of the subject.
The scientific terminology for creatine is methyl guanidine-acetic acid [NH2 - C(NH) - NCH2(COOH) - CH3]. Creatine monohydrate is a natural form of amino acid that is broken down into creatine phosphate in the body. Creatine phosphate integrates the vitality bearing molecule adenosine triphosphate (ATP). ATP…
2.1 Preparation of inoculum
The hydrogen producing anaerobic sludge containing mixed microflora was collected from the bottom of the anaerobic pond located at Felda Palm Oil Industry, Lepar Hilir, Gambang, Malaysia. To enrich the sludge with hydrogen producing bacteria and inhibit the bioactivity of methanogens, the sludge was heat treated at 90°C for 60 minutes . The treated anaerobic sludge was used as inoculum for the hydrogen production. The acclimated inoculum has a 4.6 g-SS/L…