Amperometric titration

Lactate dehydrogenase was assayed according to the method of King (1965) [21]. Reagents required: 1. 0.1 M glycine buffer: 7.5 g of glycine and 5.85 mg of sodium chloride were dissolved in one litre of distilled water. 2. Buffered substrate: 2.76 g of lithium lactate was dissolved in 125 ml of glycine buffer containing 75 ml of 0.1 N sodium hydroxide solution. This was prepared just before use. 3. 0.4 N NaOH. 4. 5.0 mg of NAD+ was dissolved in 1.0 ml of distilled water just before use. 5.…

Freezing salt-water experiment Contents: Contents, introduction and literature review on page 1. Aim, hypothesis, variables, risk assessment, materials and method on page 2. Results on page 3. Discussion and conclusion on page 4. Introduction: I have decided to do my SRP on salt water freezing because our planet is 71% water and about 96.5% of it is in the ocean which it is salt water so this is why I was interested in seeing how much salt, depresses the water from freezing. Literature…

Preformulation Study Preparation of calibration curve of Amoxicillin trihydrate [51,53,76] Preparation of stock for Hydrochloric acid Stock solution in 0.1N Hydrochloric acid (0.1N HCl): 10 mg of Amoxicillin was accurately weighed and transferred into 10 ml volumetric flask. It was dissolved and diluted to volume make up to mark with 0.1N HCl to give stock solution containing 1000 µg/ml, after than 1ml take to from 1000 µg/ml and dilute to volume make up to mark of 10ml volumetric flask with…

The beginning of the lab contained a substantial amount of information detailing what chemical properties make up the structure of a salt hydrate. It provided essential knowledge proceeding to the successful completion of this lab such as the idea that hydrates are salt compounds which have formed weak bonds to water molecules that can easily be broken through the use of heat. With this information in mind, it was expected that a series of procedural guidelines following this information be used…

The goal of this experiment was to reduce Camphor into Borneol and Isoborneol using Sodium Borohydride. In this reaction, Isoborneol was the major product while Borneol was the minor product. This is a reduction reaction, where the ketone on the Camphor is reduced to form two products with secondary alcohols. First, 0.25 grams of Camphor was obtained and added to a 50ml flask and mixed with 1.5 ml of methanol. Second, 0.25 grams of Sodium Borohydride was added to the reaction mixture and…

hydroxide (NaOH), which is a strong base. By using the back titration technique, the amount…

The unknown base concentration of a sodium hydroxide solution can be determined by titration potassium hydrogen phthalate (KHP) with an unknown concentration of sodium hydroxide NaOH. The average molarity of NaOH determine by the moles and volume of the KHP and NaOH. (Eq 1). KHC8H4O4(aq) + NaOH(aq) = H2O(l) + NaKC8H4O4(aq) (Eq 1) The unknown concentration of white vinegar can be determined by the white vinegar with the known concentration of…

Introduction This accuracy lab of titration report experiment will discuss how to find the unknown substance molarity. By using some of the lab equipment to help guide and identify the molarity of the unknown substance. I believe that it will take around three hundred drops of sodium hydroxide (NaOH) for it to be able to reach to its endpoint of the unknown substance. My reason behind my hypothesis is… Methods In this lab experiment we use a buret to…

REFLECTIONS FOR LAB 4- FRICTION Lab 4.1 to 4.3 is about all the different frictions. We did 3 different labs. 4.1 was pulling a block across different surfaces and finding the difference in friction. 4.2 was about pulling multiple blocks across different surfaces and finding the difference in friction. 4.3 was about pulling blocks but in a bigger surfaces area. In Lab 4.1 we had to pull different blocks across different surfaces. The surfaces were a tabletop, wax paper, paper towel, fine…

For this experiment, a group was employed by the Environmental Protection Agency as analytical chemists. Their goal was to identify an unknown compound that has been discovered in a landfill in their hometown. Once they identified the compound, they would then have to determine both the chemical and physical properties of the compound in order to inform the townspeople of any potential dangers. During the first week of lab, the group was given a small bottle containing an unknown white…