Amperometric titration

Abstract For this experiment the amount of zinc in vitamin water had to be determined. To do this, three methods were applied. The first was by a calibration plot method, where zinc standards were prepared and from the absorbance of those standards a calibration curve was plotted, which in turn was used to determine the amount of zinc in vitamin water. The second method was standard addition. For this method, an un-spiked and spiked solution were prepared. The un-spiked solution only contained…

(aq) + H2O(l) + C_8 H_4 O_4 〖^(2-)〗(aq) In this titration endpoint will not coincide with the equivalence point because at the equivalence point the color of the solution will be colorless and we cannot observe the endpoint, so, excessive drop of the base will color the solution to the pink and we consider it as the end point. The next titration with standard solution of NaOH with unknown concentration of HCl. The procedure is like previous titration of KHP with NaOH, but in this case instead of…

2. Materials and Methods: 2.1. Materials: Natural red, propidium iodide (PI), acridine orange and phosphate-buffered saline (PBS) were obtained from Sigma-Aldrich (St.Louis, MO). May–Grunwald–Giemsa stain was procured from Merck (Darmstadt, Germany). Fetal calf serum, Dulbecco's Modified Eagle Medium (DEMEM) and RPMI 1640 were bought from GIBCO/Life Technologies Inc. (Gaithersburg, MD). 2.2. Isolation and Proliferation of MSCs Bone marrow derived MSCs were isolated as described previously…

Measuring the mass of Iron in an iron sulphate tablet using REDOX titration Aim The aim of this experiment is to determine the mass of iron present in one iron sulphate tablet using REDOX titration under acidic conditions. Safety H2SO4: This is an irritant which may cause burns on skin and eyes; therefore care must be taken when handling this chemical. Protective gear (gloves, lab coat and safety goggles) must be worn at all times. If it comes in contact with skin or eyes, it should be…

Radioactivity past paper assignment Question 1: In the fridge, from a previous experiment, there is a 50 mM [U-14C] glucose solution. A 20 uL aliquot was counted and gave 200 000 dpm. What is the specific activity of this preparation? 20 uL = 200 000 dpm (divide both sides by 20) 1 uL = 10 000 dpm Glucose = 50 mM = 50 mmol / uL = 50 umol/ uL = 50 nmol / uL Therefore in one uL = 50 nmol/ uL = 10 000 dpm (divide both side by…

Introduction: Kidney stones are an extremely basic urinary issue. Also known as renal calculi, kidney stones are characterized by their high occurrence and a high repeat rate. This issue results from the arrangement of insoluble salt stores in the urinary tract and inside the kidney. The rate of this issue has expanded in the course of recent years for reasons that are not entirely known, but have caught the consideration of numerous scientists. There are different types of kidney stones, each…

Project 3 Identification and Synthesis of an Unknown Ionic Compound Mitchell Morgan CEM-161-007 Josh Ward 28th October 2015 Abstract The chief objective of this experiment was to identify the unknown compound. The following tests were conducted to eventually establish the identity of the compounded: qualitative solubility tests, anion and cation tests, flame test, and the formation of a precipitate. The physical properties of the unknown ionic compound found that it has no smell and was…

Observations: This experiment was divided into two parts: one part was to observe the properties of a single Alka Seltzer tablet and the second part was to find the percentage of CO2. In the first part of the experiment, the visible properties of Alka Seltzer were observed and recorded. Each Alka Seltzer contains the same three ingredients: 325 mg of Aspirin, 1000 mg of Citric Acid, and 1916 mg of Sodium Bicarbonate. Two of Alka Seltzer’s ingredients, Citric Acid and Sodium Bicarbonate (Baking…

Introduction: The purpose of this lab was to use the general chemical knowledge to identify 10 different unknown solutions. There are two main techniques that were used which are, measuring their pH, and, reacting all of the solutions together. Identifying an unknown solution is an essential skill for anyone who is going to work in a lab because many times, the label might not be accurate, or damaged. Procedure: Safety Precautions: Eye protection - Avoid skin contact. Procedure: Ten…

In the biomolecule lab, we conducted the lipid chromatography experiment to separate a mixture of biomolecules from lipids. If a positive test is conducted by the end of the experiment, there should be smudges aligned vertically on the chromatography paper, or silica gel strip, indicating the separation of different lipid molecules that were present in the substance. In contrast, if a negative test is conducted there would be no smudges aligned vertically on the chromatography paper. Substances…