Figure 5: Semi-quantitative PCR analysis of CHOP splicing variants in plasmids and liver tissues, and CHOP exon arrangements. (A) CHOP1 mRNA contains four exons, whereas CHOP2 mRNA lacks the second exon of CHOP1. The 5 '-leader of CHOP is encoded within the first and second exons, while the coding sequence is encoded within the third and fourth exons of CHOP. (B) As expected, CHOP1 primers amplified CHOP1 transcripts at the size of 214 bp in the CHOP1 plasmid template containing the full sequence of CHOP1. While, CHOP2 isoforms were detected at the size of 207 bp in the CHOP2 plasmid expressing the whole sequence of CHOP2. The presence of a single band amplified by the corresponding primers in each plasmid indicates the specificity of CHOP…
Differential alternative splicing human tissue network analysis of GUK1 Result Figure 4.12: Visualisation of alternative splicing gene GUK1 in human tissues. Differential alternative splicing genes between two different tissues comparison were shown on a different visualisation platform and tools in comparison with network analysis. (A) rMATS analysis. Histogram shows inclusion level ψ, per sample for each tissue comparisons. In this case, only exon 3 found as statistically alternative…
5 % decrease in Units Packed per Hour (UPH) for both singles paths. Without the CPT chase, the decrease in UPH would not exist and therefore we would be able to pack out more items, making more money. However for each item that misses a CPT, it costs, on average, $2.85 to upgrade the shipment. Because of this I performed two analyses to see the impact of our CPT Chase. The first analysis I performed was an analysis looking at the profitability of sending out additional packages vs. the potential…
towards an alternative workplace. A lot of companies are fitting their workplace to be able to move towards the alternative workplace. I will explain the need for alternative and innovation workplaces versus the traditional workplace. The alternative workplace allows an employee to work from anywhere. The technology changes over the last few years have made the alternative workplace a possibility. The technology that is mainly used is a portable laptop, smartphone, Wifi, computer security, VPN,…
has hatched. The alternative explanation was not given in this version. In this second version, the participants read a similar study, but the outcome of this study was not told. The alternative explanation was given. In this third version, the outcome was told that the gosling approached to the caged duck one it had hatched, and the alternative explanation was told in this version. In this final version, the similar outcome was told, but the alternative explanation was not…
I am becoming better at open-ended questions. I noticed that I would ask more close-ended questions while doing intakes instead of having a conversation with the clients and now I am much better at shifting away from closed-ended questions. I noticed that in the initial intervention I only asked one closed-ended question and none in the alternative statements. I also noticed that I am better at having more of a conversation-based intake with clients at my practicum site, just by rewording the…
would benefit would be those individuals responsible for providing group treatment programs. As the overcrowding situation is lessened, there would be the opportunity for larger meeting spaces, hopefully resulting in a potential rise in group participation rates. If an alternative to incarceration proves viable, perhaps these services could be offered to those populations outside of the grounds of the prison and would help to give the individuals the social context in which to apply these…
into Jackson Sawmill for new plant and equipment, since it hasn’t been upgraded for twenty years. The alternative would be to reinvest and downsize by reducing production capacity and permanently laying off half of the 200 workforce and build a new mill in Alberta. To build the new plant would be more expensive but the president is also considering the fact that Jackson…
Homeschooled education versus Public education It wouldn’t be an exaggeration to say that public education in the United States is a serious problem that keeps getting worse. It’ no surprise that public school system in a decline and more parents doubt the effectiveness of public schools by switching their children to homeschooling. Advocates of traditional public school system claim that public school is a best place for a child to get knowledge, thrive and better prepare for adult life.…
are taught. "Students who have been homes- schooled their entire academic life have higher scholastic- achievement test scores then students who have also attended other educational programs."(Neal, 2006, p. 54-104) Homeschooling is proving to be a reliable source despite its unusual approach to learning. According to HSLDA," Each year Harvard University takes up to 10 applicants who have had some home schooling."(HSLDA advocates for homeschooling, n.d.) Which means homeschooled children are…