Alkyl

order of polarity are as follows: ß------- Non-polar----------moderately polar--------Polar----------à C18< C8< C6< Phenyl < Amino <Cyano< Silica  In reverse phase chromatography, hydrophobic interaction between drug molecule and the alkyl chains on the column packing material.  Column is selected based on solubility, polarity & chemical differences among analytes and Column selected: i.e. Phenomenex Luna C18 column (250 X4.6 mm ID) , 5 μm particle…