Albumin

Cytochrome C, Myoglobin, Albumin and Hemoglobin(proteins) based off of their net charges. Materials: • Rabbit hemoglobin • Serum albumin(cow) • Myoglobin • Cytochrome C • Microtubes • Tape • Micropipets • Agarose gel • Buffer • Gel chamber • Test tubes • Test tube holders • Gloves • Macropipets Properties of Proteins: Protein Color Isoelectric Point Net Change at pH 8.6 Cytochrome C Orange 10.2 Positive Myoglobin Brown-red 7.2 Negative Hemoglobin Red 6.8 Negative Serum Albumin Blue 4.8 Very…

The purpose of this experiment was to explore the vast applications of eggs. Eggs are used as foams, binders, thickeners, and emulsifiers. The multiple uses enable eggs to be made into custards, souffles, and hard cooked or soft boiled eggs. One experiment conducted was comparing the effects of the cooking time for hard boiled eggs. Another experiment conducted was seeing the effects of cooking scrambled eggs in hot and cold oil, a double broiler, and the microwave. The result of this experiment…

Introduction: Enzymes are biological molecules that act as a catalyst to significantly speed up the rate of the chemical reactions that take place within cells without being used up in the process. According to the website, www.ducksters.com, “enzymes are special types of proteins. Like all proteins, enzymes are made from strings of amino acids. The function of the enzyme is determined by the sequence of amino acids, types of amino acids, and the shape of the string.” Along with this we need to…

Assignment 1: Discussion Assignment Discussion Question 1 What is the difference between biochemical reactions in healthcare? Biochemical reactions occur as a chemical reaction, which naturally takes place in a living organism. It happens within a cell involving a catabolic reaction (breaking down of molecules) or a anabolic reaction (building up of molecules). The process involves a diversity of biomolecules interacting with one another in order to make up a new biochemical for a particular…

It also measures the amounts of two major groups of proteins in the blood: albumin and globulin. Albumin is mainly synthesized in the liver. It helps keep the blood from leaking out of blood vessels. Albumin also assists in transporting certain medicines and other substances through the blood and it crucial for tissue growth and healing. Globulin is made up of different proteins called alpha…

The technique used in this experiment was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small…

plasma proteins. The weak acids and neutral drugs tend to bind to albumin (the range of drugs binding is the widest) which is one of the most common proteins found in blood plasma. This binding to the plasma proteins can play a significant role in determining drug distribution. This binding also plays a role in drug dynamics in that only the unbound or free drugs interact with the receptors and penetrate the cell membranes. Albumin along with others (i.e. lipoproteins, al-acid-glycoprotein) help…

resources relevant to the question. However, multiple synonyms, plurals or misspelled words can cause to miss studies (Melnyk & Fineout-Overholt, 2015). The key words used in this search, which came from the PICOT question, were: sepsis, mortality, albumin, fluid resuscitation, and colloids. The data bases used were: PubMed, Cochrane and Ebsco, which were accessed via Florida International University…

The purpose of this experiment is to learn how to prepare albumin from egg white and to understand the basics of protein fractionation using ammonium sulfate. Ovalbumin is the major protein of egg white and is fractionated by means of ammonium sulfate precipitation. The properties of the solvent system will dissolve the protein and greatly impact solubility. In total, there are four major factors that affect protein solubility: ionic strength, pH, temperature, and dielectric constant of solvents…

is why early screening and detection is of key importance to prevent CKD progressing to renal failure. There are two methods for available for screening and helping to detect individuals with CKD. Albumin Creatinine Ratio (ACR) is a urine test which estimates the amount of a type of protein, albumin, that is in your urine and the Glomerular Filtration Rate (GFR) can determine how well kidneys are functioning to remove wastes from your blood (National Kidney Foundation, 2012). GFR is the best…